摘要
目的探讨13岁以下患儿病原体感染后与外周血异型淋巴细胞增高的临床关系。方法选择2018年11月至2019年11月间本院收治的131例因各种病原体感染的患儿,且外周血涂片显微镜下阅片异型淋巴细胞计数>5%的患儿。同时选择健康体检者140例作为对照组。应用化学发光法检测EB病毒衣壳抗原IgM抗体、风疹病毒IgM抗体、巨细胞病毒IgM抗体、弓形虫IgM抗体、单纯疱疹病毒IgM抗体;用酶联免疫吸附法检测抗肺炎支原体抗体IgM;用酶联免疫吸附法检测抗肺炎衣原体抗体IgM;同时用血球仪进行血常规检测,用生化仪进行C反应蛋白检测。结果异淋阳性组白细胞(WBC)、淋巴细胞百分比(L)、单核细胞百分比(M)、C反应蛋白(CRP)分别为(6.9±0.8)×10^9/L、(61.6±1.9)%、(11.8±0.51)%、(5.6±1.9)mg/L,均高于对照组,中性粒细胞百分比(N)为(24.3±1.8)%,低于对照组,差异均有统计学意义(均P<0.05);显微镜确认131例患儿中异型淋巴细胞增高(>10%)的感染患儿中支气管肺炎(混合感染)、肺炎支原体肺炎、传染性单核细胞症的占比分别为22.9%、18.3%、12.2%。结论异型淋巴细胞阳性患儿白细胞分类以淋巴细胞和单核细胞增高为主,且处于疾病急性期;当异型淋巴细胞计数>10%,多见于支气管肺炎混合病原体感染,其次为肺炎支原体感染及EB病毒感染。异型淋巴细胞计数的显微镜检查,能为临床提供有利的诊断价值,在临床的鉴别诊断及治疗中具有重要的临床意义。
Objective To investigate the clinical relationship between the increase of heterotypic lymphocytes in peripheral blood and pathogen infection in children under 13 years old.Methods 131 children were selected,who were admitted to our hospital from November,2018 to November,2019,who were infected with various pathogens,and whose peripheral blood smears showed that the abnormal lymphocyte count>5%.140 healthy examinees during the same period were selected as a control group.The IgM antibodies of epstedrvirus capsid antigen,rubella virus,cytomegalovirus,toxoplasma gondii,and herpes simplex virus were detected by chemiluminescence.The Antibody IgM against mycoplasma pneumoniae was detected by ELISA.The Antibody IgM against chlamydia pneumoniae was detected by ELISA.At the same time,routine blood test was conducted by blood cell analyzer.C-reactive protein was detected by biochemical analyzer.Results The white blood cell count,lymphocyte percentage,monocyte percentage,and C-reactive protein were(6.9±0.8)×10^9/L,(61.6±1.9)%,(11.8±0.51)%,and(5.6±1.9)mg/L in the children positive in heterotypic lymphocyte,which were higher than those in the control group(all P<0.05).The neutrophil percentage was(24.3±1.8)%in the children positive in heterotypic lymphocytes,which was lower than that in the control group(P<0.05).Among the 131 children diagnosed by microscopy with increased heterotypic lymphocytes>10%,the proportions of bronchopneumonia(mixed infection),mycoplasma pneumoniae pneumonia,and infectious mononucleosis were 22.9%,18.3%,and 12.2%,respectively.Conclusion The children positive in heterotypic lymphocytes mainly have increase in lymphocytes and monocytes,and are in the acute stage of the disease.When the heterotypic lymphocyte count is>10%,the children are most commonly infected by mixed pathogens in bronchopneumonias,followed by mycoplasma pneumoniae and epstein-barr virus.The microscopic examination of abnormal lymphocyte count can provide beneficial clinical value and is of great clinical significance in differential diagnosis and treatment.
作者
王虹娟
刘雅琳
Wang Hongjuan;Liu Yalin(Clinical Laboratory of Hospital of Traditional Chinese Medicine,Karamay People's Hospital,Karamay 834000,China)
出处
《国际医药卫生导报》
2020年第12期1727-1730,共4页
International Medicine and Health Guidance News
