摘要
目的探讨在人胎盘绒毛膜板动脉中钙激活氯离子通道(CaCCs)的表达情况。方法采用实时荧光定量PCR法及蛋白免疫印记技术分别检测CaCCs的分子基础TMEM16A在妊娠期高血压疾病(HDCP)正常孕妇组、重度子痫前期组、早发型及晚发型重度子痫前期组中的表达情况。结果在正常组、重度子痫前期组中TMEM16A mRNA的△CT值分别为(15.15±0.81)、(13.86±0.54),两组差异有统计学有意义(P<0.05);在正常组和重度子痫前期组中TMEM16A的蛋白灰度值比值分别为(0.28±0.07)、(0.62±0.25),两组差异有统计学意义(P<0.05)。在早发型重度子痫前期组和晚发型重度子痫前期组中TMEM16A mRNA的△CT值分别为(14.11±0.70)、(12.99±0.46),两组差异有统计学意义(P<0.05);在早发型重度子痫前期组和晚发型重度子痫前期组中TMEM16A的蛋白灰度值比值分别为(0.47±0.18)、(0.75±0.29),两组差异有统计学意义(P<0.05)。结论TMEM16A在人胎盘膜板动脉中的表达重度子痫前期高于正常妊娠,且在重度子痫前期中晚发型中明显高于早发型,为进一步揭示妊娠期高血压发病机制奠定理论基础,提供药物靶向治疗研究的新思路。
Objective To investigate the expression of the calcium-activated chloride channels(CaCCs)in the human placental chorionic arteries.Methods Real-time quantitative PCR and Western blotting were used to detect the expression of the CaCCs molecular basis transmembrane protein 16A(TMEM16A)in the normal pregnant women,patients with severe preeclampsia and patients with early-onset and late-onset severe preeclampsia.Results The△CT value of TMEM16A mRNA was(15.15±0.81)in the normal pregnant women and(13.86±0.54)in the patients with severe preeclampsia,and the difference was statistically significant(P<0.05).The protein gray value of TMEM16A was(0.28±0.07)in the normal pregnant women and(0.62±0.25)in the patients with severe preeclampsia,and the difference was statistically significant(P<0.05).The△CT value of TMEM16A mRNA was(14.11±0.70)in the patients with early-onset severe preeclampsia and(12.99±0.46)in the patients with late-onset severe preeclampsia,and the difference was statistically significant(P<0.05).The protein gray value of TMEM16A was(0.47±0.18)in the patients with early-onset severe preeclampsia and(0.75±0.29)in the patients with late-onset severe preeclampsia,and the difference was statistically significant(P<0.05).Conclusion The expression of TMEM16A is higher in the patients with severe preeclampsia than in the normal pregnant women,and it is higher in the patients with the early-onset severe preeclampsia than in the patients with late-onset preeclampsia.The findings lay a theoretical foundation for further revealing the pathogenesis of pregnancy-induced hypertension and provide new ideas for the targeted treatment with drugs.
作者
兰秋丽
傅晓冬
Lan Qiuli;Fu Xiaodong(Department of Obstetrics, The Affiliated Hospital of Southwest Medical University, Luzhou 646000, China)
出处
《成都医学院学报》
CAS
2020年第3期358-362,共5页
Journal of Chengdu Medical College
