摘要
目的:观察电针对腹泻型肠易激综合征(IBS-D)大鼠肠上皮黏膜屏障功能的影响,探讨电针干预IBS-D的可能机制。方法:将SD大鼠随机分为空白组、模型组、电针组和药物组,每组10只,雌雄各半。采用慢性不可预知性温和应激联合番泻叶浸剂灌胃法建立IBS-D大鼠模型。电针组交替干预单侧"足三里""天枢""三阴交""太冲"穴,15 min/d,药物组予2.7 mg/mL匹维溴胺灌胃(10 mL·kg-1·d-1),均治疗14 d。采用内脏疼痛阈值和腹泻指数评价各组大鼠肠道症状;免疫组织化学法检测各组大鼠结肠组织紧密连接蛋白Claudin-1、Occludin表达水平;分光光度法检测各组大鼠血浆二胺氧化酶(DAO)活性。结果:与空白组比较,模型组大鼠腹泻指数及血浆DAO活性显著升高(P<0.01),内脏疼痛阈值、结肠Claudin-1及Occludin表达显著降低(P<0.01)。与模型组比较,电针组和药物组腹泻指数及血浆DAO活性显著降低(P<0.01),内脏疼痛阈值、结肠Claudin-1及Occludin表达显著升高(P<0.05,P<0.01)。结论:电针可以明显改善IBS-D大鼠腹痛、腹泻等肠道症状,其机制可能与增加肠上皮紧密连接蛋白Claudin-1和Occludin表达水平,降低肠道通透性,恢复肠上皮黏膜屏障功能有关。
Objective To observe the effect of electroacupuncture(EA)on intestinal epithelial mucosal barrier function in diarrhea-predominant irritable bowel syndrome(IBS-D)rats,so as to explore its mechanisms underlying improvement of IBS-D.Methods Forty SD rats(half male and half female)were randomly divided into control,model,EA and medication(Pinaverium Bromide,PB)groups,with 10 rats in each group.The IBS-D model was established by chronic unpredictable mild stress combined with gavage of Senna-leaf solution.EA(2 Hz/15 Hz,0.1-1 mA)was applied to unilateral"Zusanli"(ST36),"Tianshu"(ST25),"Sanyinjiao"(SP6)and"Taichong"(LR3)alternatively for 15 min,once daily for 14 days.Rats of the medication group was treated by gavage of PB(10 mL·kg-1·d-1)for 14 days.The visceral sensitivity(pain)was assessed by using the pressure threshold which the inserted rectal balloon catheter air-inflation(connected to a blood pressure gauge)induced stronger abdominal muscular contraction to force the rat’s abdomen to lift the experimental stand surface.The diarrhea index was used to evaluate loose stool grade.The expression of Claudin-1 and Occludin(intestinal epithelial tight junction proteins)of colon tissue was detected by immunohistochemistry.The activity of plasma diamine oxidase(DAO)was assayed by using spectrophotometry.Results Compared with the control group,the diarrhea index and plasma DAO activity in the model group were significantly increased(P<0.01),while the visceral pain threshold,expression of Claudin-1 and Occludin in the model group were significantly decreased(P<0.01).After the treatment,the diarrhea index and plasma DAO activity were significantly lower in both EA and medication groups than that in the model group(P<0.01),and the visceral pain threshold and expression levels of Claudin-1 and Occludin were obviously increased(P<0.05,P<0.01).No significant differences were found between the EA and medication groups in all the above-mentioned indexes(P>0.05).Conclusion Electroacupuncture can significantly improve abdominal pain and diarrhea in IBS-D model rats,which may be closely associated with its effects in up-regulating the expression of intestinal epithelial tight junction proteins Claudin-1 and Occludin to restore the function of intestinal epithelial mucosal barrier.
作者
姚俊鹏
赵妍
陈颖
陈丽萍
封秀梅
李瑛
周思远
YAO Jun-peng;ZHAO Yan;CHEN Ying;CHEN Li-ping;FENG Xiu-mei;LI Ying;ZHOU Si-yuan(College of Acupuncture-moxibustion and Tuina,Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China;Department of Pain,Shenzhen People’s Hospital,Shenzhen 518020,Guangdong Province)
出处
《针刺研究》
CAS
CSCD
北大核心
2020年第5期357-362,共6页
Acupuncture Research
基金
国家自然科学基金项目(No.81503665)
成都中医药大学杏林学者项目(No.QNXZ2018035)。
