摘要
采用鱼明胶分散体系制备纳米硒颗粒(SeNPs),利用动态光散射法、扫描电镜对其粒径、消化稳定性以及形貌进行表征,并对其体外抗氧化能力进行测定。结果表明:鱼明胶可作为SeNPs制备的良好分散体系,SeNPs形貌、粒径大小不受鱼明胶分散体系浓度的影响。1.000%,0.500%,0.125%鱼明胶体系制备的SeNPs表现出良好的体外抗氧化能力,其中DPPH自由基清除IC 50值分别为1.8460,0.9230,<0.4615 mg/mL;ABTS自由基清除IC 50值分别为0.5800,0.3871,<0.1935 mg/mL;不同浓度鱼明胶分散体系制备SeNPs还原力依次为0.125%鱼明胶体系>0.500%鱼明胶体系>1.000%鱼明胶体系。SeNPs在胃消化过程中会出现短暂的团聚现象,在肠消化过程中又会重新恢复纳米尺寸。
Selenium nano particles(SeNPs)were prepared using fish gelatin dispersion system,and its particle size,morphology,and digestive stability were studied by dynamic light scattering(DLS)and scanning electron microscopy(SEM).The antioxidant capacity of SeNPs were determined.The results showed that fish gelatin was excellent dispersion system for preparation of SeNPs.The particle size of SeNPs and its morphology was barely affected by the concentration of fish gelatin dispersion system.Moreover,SeNPs presented good antioxidant ability.Among them,the DPPH radical scavenging ability of SeNPs prepared by 1.000%,0.500%,0.125%fish gelatin dispersion system,and IC 50 value were 1.8460,0.9230,<0.4615 mg/mL,respectively.Correspondingly,the ABTS radical scavenging ability of SeNPs IC 50 value were 0.5800,0.3871,<0.1935 mg/mL,respectively.It was found during the in vitro simulated digestion that gastric digestion would lead to agglomeration of SeNPs,and the agglomerated selenium particles regained nano-size during intestinal digestion.
作者
都鹏程
王辉
胡月明
涂宗财
DU Peng-cheng;WANG Hui;HU Yue-ming;TU Zong-cai(State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang,Jiangxi 330047,China;National R&D Center for Freshwater Fish Processing〔Nanchang〕,Jiangxi Normal University,Nanchang,Jiangxi 330022,China;Engineering Research Center for Freshwater Fish High-value Utilization of Jiangxi Province,Jiangxi Normal University,Nanchang,Jiangxi 330022,China)
出处
《食品与机械》
北大核心
2020年第4期45-50,共6页
Food and Machinery
基金
国家自然科学基金地区科学基金项目(编号:31860428)
国家现代农业产业技术体系建设专项(编号:CARS-45)。
关键词
纳米硒
动态光散射
扫描电镜
抗氧化
消化
SeNPs
dynamic light scattering
scanning electron microscopy
antioxidant capacity
digestion
