摘要
A simple, reproducible and reliable HPLC fingerprint analysis method of Ziziphi Spinosae Semen(ZSS) and Ziziphi Mauritianae Semen(ZMS) was established, and then it was applied to analyze samples collected from different locations. A total of 12 common fingerprint peaks were designated in ZSS and ZMS, respectively, and five of which were definitely identified. Of these common peaks, 10 peaks were presented in both ZSS and ZMS, whereas two were characteristic for ZSS and ZMS, respectively. Furthermore, the two characteristic peaks of ZMS were definitely assigned as 6′′′-sinapoylspinosin and frangufoline by HPLC guided purification and structural elucidation. The HPLC fingerprint analysis was proved to be useful in identifying and discriminating ZSS and ZMS, which was beneficial for quality control of ZSS and its products.
本文建立了一种简便、可靠、重现性好的酸枣仁和滇枣仁HPLC指纹图谱分析方法,并对不同产地的酸枣仁和滇枣仁样品进行了分析。结果显示酸枣仁和滇枣仁各有12个共有指纹峰,其中10个为酸枣仁和滇枣仁共有,2个分别为酸枣仁和滇枣仁特有,经对照品对照指认了其中5个共有指纹峰。以HPLC指纹图谱为导向,对滇枣仁的两个特有指纹峰进行纯化分离和结构解析,鉴定其结构分别为6′′′-芥子酰斯皮诺素和酸枣仁碱A。该HPLC指纹图谱分析方法能够快速、准确地区分和鉴别酸枣仁和滇枣仁,可用于酸枣仁的质量控制。
基金
National Project for Standardization of Chinese Material Medica(Grant No.ZYBZH-Y-JIN-34).
