摘要
以薄荷(Mentha canadensis Linn.)cDNA为模版,克隆获得1个HD-Zip转录因子基因,对该基因的组织表达特性以及对转基因烟草(Nicotiana tabacum Linn.)叶片腺毛发育的影响效应进行分析,并对该基因编码的氨基酸序列的特性及其与其他植物的同源性和亲缘关系以及亚细胞定位和转录激活活性进行研究。结果显示:该基因被命名为McHD-Zip3,其编码区(CDS)全长2226 bp,编码741个氨基酸残基;该基因编码的氨基酸序列的理论相对分子质量为81375.67,理论等电点为pI 5.92。McHD-Zip3基因编码的氨基酸序列与其他植物HD-Zip转录因子的氨基酸序列具有一定的序列相似性,且均具有保守的Homeodomain、bZip motif、START domain和SAD domain,其与同科植物一串红(Salvia splendens Ker-Gawl.)的SsHD-Zip转录因子的序列相似度最高(82.57%)。在系统树上,McHD-Zip3转录因子与其他植物的多个腺毛发育相关的HD-Zip转录因子聚为一支,同属于HD-ZipⅣ亚家族,其与黄花蒿(Artemisia annua Linn.)的AaHD7转录因子的亲缘关系最近。McHD-Zip3转录因子定位于细胞核中,为核定位蛋白,且具有转录激活活性。McHD-Zip3基因的相对表达量在薄荷不同组织中差异显著,其在幼叶和老叶中较高,但在根和茎中较低;McHD-Zip3转基因烟草叶片的腺毛密度明显高于野生型烟草。研究结果表明:McHD-Zip3基因主要在薄荷叶片中表达,McHD-Zip3转录因子属于HD-ZipⅣ亚家族,对薄荷腺毛发育具有调控功能。
Taking cDNA of Mentha canadensis Linn.as template,a HD-Zip transcription factor gene was cloned,and its tissue expression characteristics and effect on development of glandular trichomes on leaf of transgenic Nicotiana tabacum Linn.were analyzed,meanwhile the characteristics of amino acid sequence encoded by this gene,and its homology and genetic relationship with other plants,and subcellular localization and transcriptional activation activity were studied.The results show that this gene is named as McHD-Zip3,and its full length of coding sequence(CDS)is 2226 bp,which encodes 741 amino acid residues;the theoretical relative molecular mass of amino acid sequence encoded by this gene is 81375.67,and the theoretical isoelectric point is pI 5.92.There are some sequence similarities in amino acid sequence encoded by McHD-Zip3 gene with those of HD-Zip transcription factors of other plants,and all of them possess conserved Homeodomain,bZip motif,START domain and SAD domain,and its sequence similarity with SsHD-Zip transcription factor of Salvia splendens Ker-Gawl.in the same family is the highest(82.57%).On phylogenetic tree,McHD-Zip3 transcription factor and many HD-Zip transcription factors associated with development of glandular trichomes in other plants are clustered into one branch,all of them belong to HD-ZipⅣsubfamily,and its genetic relationship with AaHD7 transcription factor of Artemisia annua Linn.is the closest.McHD-Zip3 transcription factor is a nuclear locating protein localized in cell nucleus,and being with transcriptional activation activity.The relative expression of McHD-Zip3 gene is significantly different in different tissues of M.canadensis,which is relatively high in young leaf and old leaf,but is relatively low in root and stem;the density of glandular trichomes on leaf of McHD-Zip3 transgenic N.tabacum is evidently higher than that of wild type N.tabacum.It is suggested that McHD-Zip3 gene is mainly expressed in leaf of M.canadensis,and McHD-Zip3 transcription factor belongs to HD-ZipⅣsubfamily,which has regulatory function on development of glandular trichomes of M.canadensis.
作者
陈泽群
亓希武
房海灵
于盱
李莉
梁呈元
CHEN Zequn;QI Xiwu;FANG Hailing;YU Xu;LI Li;LIANG Chengyuan(Jiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China)
出处
《植物资源与环境学报》
CAS
CSCD
北大核心
2020年第3期1-10,共10页
Journal of Plant Resources and Environment
基金
国家自然科学基金资助项目(31970353)
江苏省植物资源研究与利用重点实验室开放基金资助项目(JSPKLB201838)。
关键词
薄荷
McHD-Zip3基因
表达特性
亚细胞定位
转录激活活性
腺毛发育
Mentha canadensis Linn.
McHD-Zip3 gene
expression characteristics
subcellular localization
transcriptional activation activity
development of glandular trichome
