摘要
细胞共培养技术一般用于研究细胞与细胞间的通讯机制,对揭示多细胞生物生理和病理过程具有重要意义.基于微流控芯片的细胞共培养技术能够模拟原生微环境以进行复杂的代谢和调控,为研究细胞与细胞间通讯提供了新的共培养技术平台,已经广泛应用于肿瘤转移及分析、抗癌药物筛选、药物吸收和药物代谢等领域.本文从微流控芯片上细胞共培养系统的设计、共培养系统上的检测以及模型的应用方面进行了总结和展望.
Cell co-culture technology is generally used to study the mechanism of cell-cell communication,which is of great significance for revealing multi-cellular biological and pathological processes.The microfluidic chip-based cell co-culture technology can simulate the native microenvironment for complex metabolism and regulation,and provides a new co-culture technology platform for studying cell-cell communication.It has been widely used in tumor metastasis and analysis,and anti-cancer drug screening,drug absorption and drug metabolism.In this paper,the design of the cell co-culture system on the microfluidic chip,the detection on the co-culture system and the application of the model are summarized and prospected.
作者
段肖肖
刘思秀
DUAN Xiaoxiao;LIU Sixiu(Department of Environmental Science and Engineering, Fudan University, Shanghai 200438, China;Shanghai Key Laboratory of Atmospheric Particle Pollution Prevention, Fudan University, Shanghai 200438, China)
出处
《复旦学报（自然科学版）》
CAS
CSCD
北大核心
2020年第2期245-252,共8页
Journal of Fudan University：Natural Science
基金
国家自然科学基金(21577020,21876031)。
