摘要
通过筛选耐受高浓度Ca2+且钙化速率较高的藻株,分析初始Ca2+浓度对其生长和钙化效果的影响。从喀斯特地区湖泊水样分离得到多株微藻,筛选出钙化能力较高的斜生栅藻(Scenedesmus obliquus) B-7。收集藻细胞并进行扫描电镜观察和能谱仪(SEM-EDS)分析,发现细胞表面存在针状霰石形貌的钙化结晶,具有明显的钙化现象。进一步在不同Ca2+浓度下培养藻株B-7,测定细胞生长和培养基pH随时间的变化情况及Ca2+消耗量,结果表明,初始Ca2+浓度与细胞生长呈负相关。从细胞生长和生物钙化量综合考虑,400 mg/L Ca2+为藻株B-7进行生物钙化提供了适宜的环境;在此质量浓度下,生物钙化程度最大,生物钙化率达到86. 09%。
This work aims to screen out microalgal strains with strong Ca2+-resistant ability and high calcification rate,and investigate the effects of initial Ca2+concentration on the growth and calcification of the selected microalgal strains.Several microalgal strains are isolated from water samples of Karst regions.Among them,Scenedesmus obliquus B-7 exhibits the highest calcification rate in Ca2+-rich media. Microalgal cells are collected,and analyzed by scanning electron microscope and energy disperse spectroscopy( SEM-EDS).It is found that calcification crystals with needle-like aragonite morphology are observed on the cell surface,which implies an in vivo calcification phenomenon.Furthermore,B-7 microalgal strain is cultured under a series of initial Ca2+concentrations. The changes of cell growth and medium pH with time,and Ca2+consumption are measured. It is found that cell growth is negatively correlated with initial Ca2+concentration.In consideration of both cell growth and biocalcification,the optimum initial Ca2+concentration is 400 mg·L-1,in which the highest biocalcification proportion of 86. 09% can be obtained.
作者
赵前
冉朝刚
赵靖文
张永奎
谢通慧
ZHAO Qian;RAN Chao-gang;ZHAO Jing-wen;ZHANG Yong-kui;XIE Tong-hui(Sichuan University,Chengdu 610065,China)
出处
《现代化工》
CAS
CSCD
北大核心
2020年第4期119-123,128,共6页
Modern Chemical Industry
基金
国家自然科学基金青年科学基金项目(21506132)
四川大学“化工之星”优秀青年人才培养计划(2019-2020)。
关键词
钙化微藻
生物矿化
钙化率
生长
筛选
calcified microalgae
biomineralization
calcification rate
growth
screening
