摘要
查尔酮合成酶(Chalcone synthase,CHS)广泛存在于植物体内,是花色素形成过程中一种重要的酶,可以进一步催化生成黄酮类化合物。本研究采用Codon W和EMBOSS在线软件对红松查尔酮合成酶基因CHS的密码子使用偏好性进行分析,并与北美乔松等其他24种植物的CHS基因以及模式植物基因组进行比较,对认识红松CHS基因的密码子使用偏好性,为选择适宜的表达系统奠定了一定的基础。研究结果表明:红松CHS基因编码区的有效密码子数(ENC)和GC含量分别为48.92和0.548,C+G含量高于A+T含量,密码子偏好以A/T结尾;多数植物CHS基因的G+C含量高于A+T含量,且密码子更偏好C/G结尾;聚类分析表明,红松与马尾松和赤松的密码子使用偏好性的相似性较高;密码子使用频率研究发现,红松CHS遗传转化与异源表达较优的受体可能是大肠杆菌和拟南芥。
Chalcone synthase( CHS) is widely found in plants and is a key enzyme in anthocyanin synthesis pathway. It can catalyze the binding of malonyl coenzyme A and coumaryl coenzyme A to generate flavonoids.CodonW and EMBOSS were used to analyze the codon usage bias of CHS gene in Pinus koraiensis. The CHS gene of P. koraiensis were compared with those of the other 24 plants and model plant genome,which laid a foundation for the selection of appropriate expression system. The effective codon number( ENC) and GC content of CHS gene coding region were 48.92 and 0.351,respectively. The content of C + G was higher than that of A + T,and the codon preferred to end with A/T. The G + C content of most plants CHS genes is higher than that of A + T,and the codon preferred to end with C/G. By cluster analysis,the codon usage bias of P. koraiensis was similar to P. massoniana and P. densiflora pdchsx. In addition,it was found that Arabidopsis thaliana and Escherichia coli might be ideal receptor systems for CHS genetic transformation and heterogeneous expression.
作者
李翔
范作义
王井源
王淇
李喜鹏
王德秋
孔令远
曹森林
孟庆刚
赵曦阳
LI Xiang;FAN Zuo-Yi;WANG Jing-Yuan;WANG Qi;LI Xi-Peng;WANG De-Qiu;KONG Ling-Yuan;CAO Sen-Lin;MENG Qing-Gang;ZHAO Xi-Yang(State Key Laboratory of Tree Genetics and Breeding,School of Forestry,Northeast Forestry University,Harbin 150040;Linjiang Forestry Bureau of Jilin Province,Linjiang 134600)
出处
《植物研究》
CAS
CSCD
北大核心
2020年第3期447-457,共11页
Bulletin of Botanical Research
基金
红松种质资源遗传多样性评价项目(KJZXSA2019046)。
关键词
红松
查尔酮合成酶
密码子偏好性
聚类分析
Pinus koraiensis
chalcone synthase
codon usage bias
cluster analysis
