摘要
目的观察痰瘀同治法通过调节TGF-β1/Smad3信号通路对糖尿病大鼠肾脏病变的影响。方法选取SD大鼠随机分为:空白组(NC)、模型组(MC)、痰瘀同治组(RPDBS)、化痰组(RP)、化瘀组(DBS),予以单次注射链脲佐菌素溶液制备糖尿病模型,喂养4周后,RPDBS组、RP组和DBS组分别予以相应的药物干预8周,麻醉状态下取材。采用HE染色法观察肾脏组织病理学变化;Western blot法和实时荧光定量PCR法检测肾脏组织转化生长因子-β1(TGF-β1)及其信号蛋白Smad3蛋白和基因表达水平。结果与MC组比较,光镜下各治疗组肾小球和肾小管结构和排列改善明显;各治疗组TGF-β1、Smad3和p-Smad3蛋白和基因表达水平均明显下调(P<0.05);其中RPDBS组TGF-β1、Smad3和p-Smad3蛋白和基因表达水平较RP组和DBS组下调更明显(P<0.05)。结论痰瘀同治可能通过抑制TGF-β1/Smad3信号通路激活,延缓糖尿病肾病纤维化,发挥肾脏保护作用。
Objective To observe the effect of traditional Chinese medicine for intervention of phlegm and blood stasis in regulating TGF-β1/Smad3 signaling and relieving nephropathy in diabetic rats.Methods SD rats were divided into blank group(NC),diabetic model group(MC group),intervention of phlegm and blood stasis(RPDBS)group,phlegm-removing(RP)group and blood-removing(DBS)group.Diabetic models were established in all the rats except for those in the blank group.After 4 weeks of feeding,the rats in RPDBS group,RP group and DBS group were given corresponding drug intervention for 8 weeks.HE staining was used to observe the changes in renal histopathology.Western blotting and real-time fluorescence quantitative PCR were used to detect the expression levels of transforming growth factor-β1(TGF-β1)and Smad3.Results The structure and arrangement of the glomeruli and renal tubules improved significantly in the treatment groups in comparison with those in the MC group.The expression levels of TGF-β1,Smad3 and p-Smad3 were significantly downregulated at both the protein and mRNA levels in the treatment groups(P<0.05),and the down-regulation was more obvious in RPDBS group than in RP group and DBS group(P<0.05).Conclusion Intervention of phlegm and blood stasis may inhibit the activation of TGF-β1/Smad3 signaling pathway and delay diabetic nephropathy and fibrosis to protect the renal function in diabetic rats.
作者
储全根
蔡正银
储俊
轩云
程婕
王盼
CHU Quangen;CAI Zhengyin;CHU Jun;XUAN Yun;CHENG Jie;WANG Pan(College of Traditional Chinese Medicine,Anhui University of Chinese Medicine,Hefei 230012,China;Key Laboratory of Xin'an Medicine of Ministry of Education,Anhui University of Chinese Medicine,Hefei 230012,China)
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2020年第5期708-712,共5页
Journal of Southern Medical University
基金
国家自然科学基金(81774189)
安徽省中医药领军人才项目,新安医学教育部重点实验室项目。
