摘要
目的:研究microRNA-24(miR-24)能否作为急性动脉粥样硬化性脑梗死进展的生物标志物。方法:选取2018年6月—2019年5月我院神经内科收治的急性动脉粥样硬化性脑梗死患者194例,根据有无脑梗死进展(Progressive cerebral infarction,PCI)分为进展组60例和对照组134例。收集两组患者的基线特征及常见脑梗死高危因素。两组使用定量RT-PCR测定miR-24的表达。使用酶联免疫吸附试验检测血清hs-CRP和IL-6的表达。结果:与对照组相比,进展组脑梗死后24h内血清miR-24表达显著增加。血清miR-24的上调与血清hs-CRP、梗死体积和美国国立卫生研究院脑梗死量表(NIHSS)评分呈显著正相关,与患者血清IL-6呈中度正相关。血清hs-CRP和血清miR-24的组合对于PCI的预测(AUC:0.782~0.863)具有更好的灵敏度/特异性。结论:血清miR-24是评估急性动脉粥样硬化性脑梗死进展的潜在生物标志物。
Objective:To investigate whether microRNA-24 can be used as a biomarker for the progression of acute atherosclerotic cerebral infarction.Methods:From June 2018 to May 2019,194 patients with acute atherosclerotic cerebral infarction admitted to the department of neurology in our hospital,according to the progress of cerebral infarction(PCI),there were 60 patients in the progression group and 134 patients in the control group.Baseline characteristics of the two groups of patients and common risk factors for cerebral infarction were collected.Quantitative RT-PCR was used to determine the expression of miR-24 in both groups.Serum hs-CRP and IL-6 were detected by enzyme-linked immunosorbent assay.Results:Serum miR-24 expression was significantly increased within 24 hours after cerebral infarction in the progressive group compared with the control group.The up-regulation of serum miR-24 was positively correlated with serum hs-CRP,infarct volume,and National Institutes of Health Cerebral Infarction Scale(NIHSS)scores,and was moderately positively correlated with serum IL-6.The combination of serum hs-CRP and serum miR-24 has better sensitivity/specificity for PCI prediction(AUC:0.782~0.863).Conclusion:Serum miR-24 is a potential biomarker for assessing the progression of acute atherosclerotic cerebral infarction.
作者
袁磊
赵晓玲
王明
刘洋
鹿滨麒
黄鹂丽
YUAN Lei;ZHAO Xiaoling;WANG Ming(Department of Neurology, the Second People’s Hospital of Nanyang City, He’nan Province 473000)
出处
《医学理论与实践》
2020年第10期1561-1564,共4页
The Journal of Medical Theory and Practice
