摘要
目的 建立三七HPLC指纹图谱,并测定5种成分的含有量.方法 三七70%甲醇提取物的分析采用Waters XBridge C18色谱柱(4.6 mm×250 mm,5μm);流动相乙腈-水,梯度洗脱;体积流量1.5 mL/min;柱温25℃;检测波长203 nm.结果 15批样品指纹图谱中有5个共有峰,相似度均大于0.99.三七皂苷R1、人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1和人参皂苷Rd分别在0.000 76~0.567 64(r=0.9999)、0.002 69~2.017 43 (r=0.9998)、0.000 38~0.283 82 (r=1.000 0)、0.002 83~2.12483 (r=0.999 8)、0.000 78~0.582 98 mg/mL (r=0.999 8)范围内线性关系良好,平均加样回收率分别为101.78%、97.22%、102.14%、98.96%、101.73%,RSD分别为1.58%、1.31%、2.17%、1.55%、1.80%.结论 该方法简便、快速、准确,可用于三七的质量控制.
AIM To establish the HPLC fingerprints of Panax notoginseng(Burk.)F.H.Chen and to deter mine the contents of five constituents.METHODS The analysis of the 70%methanol extract from P.notoginseng was developed on a 25℃thermostatic Waters XBridge C18 column(4.6 mm×250 mm,5μm),with the mobile phase comprising of acetonitrile water flowing at 1.5 mL/min in a gradient elution manner,and the detection wave length was set at 203 nm.RESULTS There were five common peaks in the fingerprints of fifteen batches of sam ples,with the similarities of more than 0.99.Notoginsenoside R1,ginsenoside Rg1,ginsenoside Re,ginsenoside Rb1and ginsenoside Rd showed good linear relationships within the ranges of 0.00076 0.56764(r=0.9999)、0.00269 2.01743(r=0.9998)、0.00038 0.28382(r=1.0000)、0.00283 2.12483(r=0.9998)、0.00078 0.58298 mg/mL(r=0.9998),whose average recoveries were 101.78%,97.22%,102.14%,98.96%,101.73%with the RSDs of 1.58%,1.31%,2.17%,1.55%,1.80%,respectively.CONCLUSION This stable and reliable method can be used for the quality control of P.notoginseng.
作者
李宁
高小惠
王子幼
龚云麒
刘军锋
杨兆祥
崔秀明
王峥涛
LI Ning;GAO Xiao hui;WANG Zi you;GONG Yun qi;LIU Jun feng;YANG Zhao xiang;CUI Xiu ming;WANG Zheng tao(KPC Pharmaceuticals,Inc,Kunming 650100,China;The MOE Key Laboratory for Standardization of Chinese Medicines,Institute of Chinese Ma teria Medica,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Key Laboratory of Sustainable Utilization of Panax Notoginseng Resources of Yunnan Province,Kunming 650500,China)
出处
《中成药》
CAS
CSCD
北大核心
2020年第5期1232-1237,共6页
Chinese Traditional Patent Medicine
基金
国家中药标准化项目(ZYBZH C YN 58)
云南省应用基础研究计划项目(2018FB141)
云南省重大科技专项(2017ZF001)
云南省三七资源可持续利用重点实验室开放项目(2018)。
