摘要
目的运用网络药理学筛选骨碎补有效成分治疗骨质疏松症的作用靶标及信号通路。方法首先借助TCMSP分析平台、GeneCards数据库和OMIM数据库,分别筛选中药活性成分、疾病及相应的靶标,将中药靶标与疾病靶标取交集,得到中药-疾病共同靶标并构建中药-疾病-靶标调控网络。然后通过构建中药-疾病共同靶标的蛋白互作网络,筛选核心靶点基因。最后将中药-疾病共同靶标进行GO功能富集分析和KEGG功能富集分析,得出中药-疾病共同靶标相关的信号通路。结果筛选得到骨碎补治疗骨质疏松症的18个有效成分和98个中药-疾病共同靶标,并推断其作用机制可能与糖尿病并发症的AGE-RAGE信号通路、TNF信号通路、IL-17信号通路、HIF-1信号通路、Th17细胞分化信号通路等有关,且白介素-6基因、JUN基因、丝裂原活化蛋白激酶-1基因和表皮细胞生长因子基因可能起着关键性作用。结论基于网络药理学探讨了骨碎补治疗骨质疏松症的作用靶标及信号通路,为中药现代化研究提供了思路。
Objective To screen the target and signaling pathway of the effective ingredients of Rhizoma Drynariae in the treatment of osteoporosis by network pharmacology.Methods Firstly,TCMSP analysis platform,GeneCards database and OMIM database were used to screen the active ingredients and corresponding targets of Traditional Chinese Medicine(TCM),and the common target of TCM-Disease was obtained by intersecting the target of TCM with the target of disease,and the regulatory network of TCM-Disease-Target was constructed.Then,the core target genes were screened by constructing the protein protein interaction(PPI)network of common target of TCM-Disease.Finally,GO function enrichment analysis and KEGG function enrichment analysis were carried out on the common target of TCM-Disease,and the signal pathways related to the common target of TCM-Disease were obtained.Results 18 effective ingredients and 98 common targets of TCM-Disease were screened for the treatment of osteoporosis.The mechanism of action may be related to AGE-RAGE signaling pathway,TNF signaling pathway,IL-17 signaling pathway,HIF-1 signaling pathway,Th17 cell differentiation signaling pathway,and interleukin-6 gene,JUN gene,mitogen-activated protein kinase-1(MAPK-1)gene and epidermal growth factor(EGF)gene may play a key role.Conclusion Based on network pharmacology,the target and signal pathway of Rhizoma Drynariae in the treatment of osteoporosis were explored,which provides ideas for the modernization of TCM.
作者
马江涛
万雷
黄宏兴
MA Jiangtao;WAN Lei;HUANG Hongxing(Guangzhou University of Chinese Medicine,Guangzhou,Guangdong 510405,China;The Third Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou,Guangdong 510375,China)
出处
《中国骨质疏松杂志》
CAS
CSCD
北大核心
2020年第4期490-496,共7页
Chinese Journal of Osteoporosis
基金
国家自然科学基金项目(81674004,81673786)
广东省科技计划项目(2016A020216024)
广州中医药大学学科研究重点项目(XK2019028)。
关键词
网络药理学
骨碎补
骨质疏松症
信号通路
network pharmacology
rhizoma drynariae
osteoporosis
signaling pathway
