摘要
前交叉韧带(anterior cruciate ligament, ACL)重建术后腱骨止点愈合质量直接影响临床效果。前期实验证实三七总皂苷(panax notoginseng saponins,PNS)可显著改善重建ACL腱骨愈合处成骨能力,可能与移植物内源性肌腱干细胞(tendon derived stem cells,TDSCs)的成骨分化密切相关。探讨了PNS对TDSCs成骨分化能力的影响。将培养至第3代的大鼠TDSCs分为PNS组与对照组2组,利用CCK-8实验方法检测PNS对细胞活性的影响;TDSCs经PNS处理72 h后加入成骨培养基进行诱导分化,检测成骨标志物碱性磷酸酶的表达情况;在裸鼠皮下注射PNS处理后的TDSCs,4周后利用Micro-CT检测异位成骨情况。研究证实PNS可显著增强TDSCs细胞成骨分化与裸鼠异位成骨能力。
The anterior cruciate ligament(ACL) is an important structure of the knee, easily getting hurt. Currently, it is a common practice using the autologous tendon graft to reconstruct the ACL and to treat the ACL injury. However, the tendonbone interface heals slowly after the ACL reconstruction, which affects the early rehabilitation of the patients. The Panax Notoginseng Saponins(PNS), a blood-activating and blood-stasis drug, is often used for the treatment of fractures. Our previous experiments show that the PNS can significantly enhance the osteogenesis effect of the tendon autograft in the reconstruction of the ACL. Thus, this paper focuses on the effect of the PNS on the osteogenic differentiation of the tendon derived stem cells(TDSCs). The TDSCs of passage 3 are divided into two groups, the PNS group and the negative control group. The effect of the PNS on the cell activity is detected by the flow cytometry and the CCK8. After 72 hours of the PNS treatment, the TDSCs are cultured with the osteogenic medium, and the expression of the osteogenic marker ALP is detected by the ALP staining and the Elisa. The TDSCs are subcutaneously injected into nude mice after the PNS treatment, and then the micro-CT is used to detect the ectopic osteogenesis 4 weeks later. Compared with the negative control group, it is shown that the PNS could promote the osteogenic differentiation of the TDSCs and the ectopic osteogenesis in nude mice.
作者
刘晓华
孙晋
马佳
张晟
姜博
李妍
张磊
LIU Xiaohua;SUN Jin;MA Jia;ZHANG Sheng;JIANG Bo;LI Yan;ZHANG Lei(Department of Joint Surgery and Sports Medicine,Wangjing Hospital,China Academy of Chinese Medical Sciences,Beijing 100102,China)
出处
《科技导报》
CAS
CSCD
北大核心
2020年第6期41-45,共5页
Science & Technology Review
关键词
三七总皂苷
肌腱干细胞
成骨分化
panax notoginseng saponins
tendon derived stem cells
osteogenic differentiation
