摘要
目的调查江西省环鄱阳湖地区蜱虫和动物宿主蜱传病原体携带情况。方法采用PCR扩增和测序法确定病原体种类。结果对采集的311份蜱虫,452份宿主血样标本(162份犬血、166份羊血、105份人血和19份鼠血)进行蜱传病原体检测。检测的犬中有7.41%感染巴贝西虫,1.85%感染支原体Mycoplasma spp.,鼠有31.58%感染无形体,人和羊未能检测到蜱传病原体;蜱虫有7.07%感染病原体,长角血蜱和褐黄血蜱合并感染了立克次体Rickettsia spp.和原虫。褐黄血蜱病原体携带率高,而长角血蜱偏低。成蜱感染率高于幼若虫(χ^2=12.48,P<0.001),叮咬刺猬的蜱虫感染率高于叮咬其他宿主的蜱虫(χ^2=108.44,P<0.001),基因测序和分析表明,在检测的蜱虫中至少发现了4种病原体,即扬子江螺旋体、斯洛伐克立克次体或劳氏立克次体相关基因型、伏氏巴贝西虫以及犬肝族虫或猫肝族虫相关基因型。结论本文首次报道了江西省环鄱阳湖地区蜱传病原体在蜱虫和动物宿主中分布情况,本研究将为该地区蜱虫和蜱传病原体防控提供重要参考。
Objective To determine the tick-borne pathogens in animal hosts and tick vectors.Methods Tick-borne pathogens were determined with PCR amplification followed by sequencing.Results 311 ticks and 452 blood samples from 162 dogs,166 goats,105 humans and 19 rodents were collected for tick-borne pathogen determination.As a result,7.41%and 1.85%of dogs were detected positive for Babesia spp.,and Mycoplasma spp.respectively.In addition,the rate of Anaplasma spp.infection was 31.58%in rodents.However,humans and goats were negative for Borrelia spp.,Coxiella spp.,Ehrlichia spp.,Rickettsia spp.,Anaplasma spp.,Mycoplasma spp.,Babesia spp.,and Hepatozoon spp.Further sequence analyses verified Mycoplasma haemocanis,Babesia gibsoni and Babesia vogeli in dogs and Anaplasma phagocytophilum in rodents.Of the collected ticks,7.07%were positive for tick-borne pathogens,and Haemaphysalis longicornis and Haemaphysalis flava were found to be co-infected with Ricketssia spp.and protozoan.H.flava was the most detected positive for tick-borne pathogens,whereas H.longicornis had the lowest infection rate.Furthermore,adult ticks demonstrated remarkably higher infection rate than immature ticks(χ^2=12.48,P<0.001),meanwhile ticks on Erinaceidae showed significantly higher positivity than ticks collected on other host species(χ^2=108.44,P<0.001).Genetic fragment sequencing and analyses showed at least 4 pathogen species presence in ticks,namely Borrelia yangtzensis,Rickettsia slovaca or Rickettsia raoultii related genospecies,B.vogeli and Hepatozoon canis or Hepatozoon felis related genospecies.Conclusion This is the first molecular report documenting tick-borne pathogens in hosts and ticks in Poyang Lake region of Jiangxi Province.In the future,the tick-borne pathogens detected in this study should be taken into consideration for human and animal disease control and prevention.
作者
柳小青
郑卫青
付仁龙
陶卉英
刘仰青
马红梅
陈海婴
LIU Xiao-qing;ZHENG Wei-qing;FU Ren-long;TAO Hui-ying;LIU Yang-qing;MA Hong-mei;CHEN Hai-ying(The Collaboration Unit for Field Epidemiology of State Key Laboratory for Infectious Disease Prevention and Control,Jiangxi Provincial Key Laboratory of Animal-origin and Vector-borne Diseases,Nanchang Center for Disease Control and Prevention,Nanchang 330038,China)
出处
《中华卫生杀虫药械》
CAS
2020年第2期151-157,共7页
Chinese Journal of Hygienic Insecticides and Equipments
基金
江西省科技厅项目(编号:20161BBG70005)
江西省卫计委项目(编号:20162007)
南昌市科技局项目(编号:洪科字〔2016〕96号第77项)。
