摘要
以罗丹明6G和水合肼为原料,先制备罗丹明6G酰肼,接着在乙醇中滴加少量冰醋酸做催化剂后与2,5-二甲氧基苯甲醛反应,合成了一种新型的pH荧光分子探针(RGSBD),进行了结构表征及荧光性能研究。结果表明,原本在氢离子浓度较低,即体系pH较高时(pH≥4.0),探针RGSBD内酰胺螺环闭环导致不显示荧光并且无色,然而在氢离子浓度较大即体系pH较低时(pH<4.0)时,其内酰胺螺环闭环产生了明显的颜色变化,发出强烈的荧光。pH 1.9时,探针的荧光强度达到最大,最大荧光峰发生显著的红移。进一步研究表明,探针RGSBD的荧光峰强度差值与pH在1.9~3.2范围内呈良好的线性关系,探针RGSBD识别H^+的选择性高,稳定性与可逆性强,可发展用作生物体内pH荧光传感材料。
Rhodamine 6 G and hydrazine hydrate were used as raw materials to synthesize rhodamine 6 G hydrazide.Then,a novel pH fluorescent molecular probe(RGSBD)was synthesized by rhodamine 6 G hydrazide and 2,5-dimethoxybenzaldehyde under glacial acetic acid as catalyst.This compound was characterized by1 H NMR,IR and fluorescence spectroscopy.The colorless spirocyclic structure of probe RGSBD at high pH(pH≥4.0)opened to the colored and highly fluorescent form at very low pH(pH<4.0).At pH 1.9,the fluorescence intensity of the probe RGSBD reached maximum accompanying with obvious red-shifted of the maximum fluorescence peak.Further studies showed that the fluorescence peak intensity difference of the probe RGSBD had a good linear relationship with pH in the range of 1.9-3.2.Common metal ions had almost no interference with the fluorescent response of probe RGSBD to H^+,indicating high selectivity of the probe.The probe can be promoted as a new sensing material for studying pH in biological system due to its satisfactory stability and reversibility.
作者
吴芳辉
杨俊卿
罗祥瑞
李红
朱海威
余爱民
WU Fanghui;YANG Junqing;LUO Xiangrui;LI Hong;ZHU Haiwei;YU Aimin(School of Chemistry and Chemical Engineering,Anhui University of Technology,Ma'anshan 243000)
出处
《分析试验室》
CAS
CSCD
北大核心
2020年第2期137-141,共5页
Chinese Journal of Analysis Laboratory
基金
国家自然科学基金(21771003)
安徽省自然科学基金(1808085MB36)
安徽省教育厅自然科学重点基金(KJ2015A024)
安徽省大学生创新创业训练计划项目(S201910360213)资助。
关键词
罗丹明6G
席夫碱
荧光探针
合成
应用
rhodamine 6G
schiff base
fluorescence probe
synthesis
application
