摘要
【目的】揭示日龄和交配对亚洲玉米螟Ostrinia furnacalis雄蛾内生殖器官的影响,以及亚洲玉米螟雄蛾的交配状态和性信息素诱捕之间的关系。【方法】采用行为学和生殖器解剖技术,研究不同日龄和交配状态亚洲玉米螟雄蛾内生殖器特征,并将其与田间性信息素诱捕雄蛾进行比较。【结果】除了精巢、输精管、储精囊、附腺、复射精管和单射精管外,亚洲玉米螟雄蛾内生殖系统还包含1对附腺囊,同时单射精管非角质化区分为5段(PS1-5),PS1又细分为5区(PS1Ⅰ-Ⅴ)。生殖器内含物可分为5级(0-4级),存在充满和未充满生殖器两种状态。1日龄雄蛾内生殖系统不同器官的内含物等级存在差异,但是日龄对雄蛾生殖系统内含物等级无明显影响。交配后0 h亚洲玉米螟雄蛾,除输精管、储精囊和附腺囊外,其余生殖器官内含物等级较未交配雄蛾的均发生显著变化。随着交配后时间的增加,雄蛾生殖器内含物等级逐渐恢复,各器官内含物等级恢复的速度存在差异,交配后60 h所有内含物等级均恢复到未交配状态。但是直到交配后228 h,交配雄蛾PS5内含物仍然表现出断裂等形态特征,和未交配雄蛾存在差异。性信息素引诱雄蛾中有60.9%的生殖系统表现出未交配雄蛾特征。【结论】利用PS5内含物的形态可以鉴别亚洲玉米螟雄蛾的交配状态,性信息素群集诱杀的亚洲玉米螟雄蛾大部分是未交配。本研究结果为亚洲玉米螟雄蛾交配状态的判定和蛾类害虫性信息素测报和防治的有效性提供了一定依据。
【Aim】This study aims to clarify the effects of age and mating on the internal genital organs of male adults of Ostrinia furnacalis and to ascertain the relationship between the mating status of O.furnacalis male moths and sex pheromone trapping.【Methods】The characteristics of internal genital organs of male adults of O.furnacalis at different age(day-old)and in different mating statuses were studied with behavioral bioassay and genital anatomy and compared with those of male adults captured with sex pheromone lure in the field.【Results】In addition to the testis,vas deferens,vesiculae seminales,accessory glands,ductus ejaculatorius duplexes and ductus ejaculatorius simplex,the reproductive system of male moths of O.furnacalis also contains a pair of ponds of accessory gland.The primary segment of ductus ejaculatorius simplex can be divided into five segments(PS1-5),and the segment PS1 can be divided into five regions(PS1Ⅰ-Ⅴ).The genital contents in the genital duct can be grouped into five grades(grade 0-4)according to the compactness,with two statuses of the genital duct fully and not fully filled with contents.The grades of genital contents of different genital organs in 1-day-old male moth were different,but the age had no evident effect on the grade of genital contents of the reproductive system of male moth.Except for vas deferens,vesiculae seminales and ponds of accessory gland,the grades of genital contents of other reproductive organs in the newly mated male moths were significantly different from those in the unmated individuals.With the increase of time after mating,the grades of genital contents of male reproductive organs were gradually recovered,and the recovery rates of different genital organs were different.After 60 h,the grades of genital contents of the mated males were the same as that of the unmated individuals.However,until 228 h after mating,the genital contents of PS5 in the mated male moths still showed some different morphological characteristics like breaks from those of the unmated male moths.In the male moths attracted by sex pheromone lures,the reproductive system of 60.9%males showed the same characteristics as that of the unmated male moths.【Conclusion】The characteristics of the genital content of PS5 can be used to distinguish the mated and unmated male moths of O.furnacalis,and most of male moths of O.furnacalis attracted by sex pheromone are unmated.The results of this study lay a theoretical foundation for the determination of mating status of male moths of O.furnacalis and for pest moth monitoring and control by sex pheromone trapping.
作者
冯波
张万民
张丹
屈丽莉
孟威
王巍
王浩杰
杜永均
FENG Bo;ZHANG Wan-Min;ZHANG Dan;QU Li-Li;MENG Wei;WANG Wei;WANG Hao-Jie;DU Yong-Jun(Institute of Health and Environmental Ecology,Wenzhou Medical University,Wenzhou,Zhejiang 325035,China;Liaoning Technological Center of Green Agriculture,Shenyang 110034,China;Ningbo NewCon Inc.,Ningbo,Zhejiang 315000,China;Institute of Pesticide and Environmental Toxicology,Zhejiang University,Hangzhou 310058,China)
出处
《昆虫学报》
CAS
CSCD
北大核心
2020年第3期317-326,共10页
Acta Entomologica Sinica
基金
国家自然科学基金项目(31871956,31700329)
浙江省重点研发计划(2018C02027)。
关键词
亚洲玉米螟
雄性生殖系统
交配状态
性信息素
群集诱杀
Ostrinia furnacalis
male reproductive system
mating status
sex pheromone
mass trapping
