摘要
桑粉虱(Pealius mori)是对我国蚕区桑树造成严重危害的粉虱类害虫。应用Wolbachia次级内共生菌wsp基因和Rickettsia、Cardinium、Hamlitonella次级内共生菌16S rDNA特异引物对采自田间的桑粉虱样本基因组DNA进行PCR扩增、测序,通过分析测序得到的基因序列确定次级内共生菌种类,并对桑粉虱样本感菌情况进行检测分析。桑粉虱样本Rickettsia的扩增序列(963 bp)与烟粉虱(Bemisia tabaci)样本Rickettsia扩增序列(KY620205、GU563843、FJ915603)的碱基一致性为99.90%~100%;桑粉虱样本Cardinium的扩增序列(446 bp)与烟粉虱样本Cardinium扩增序列(FJ766341、GU563845、HG421084、KT827382)的碱基一致性为99.78%;桑粉虱样本Hamlitonella的扩增序列(694 bp)与烟粉虱样本Hamlitonella扩增序列(MH908669、KM197206、AB981348、AF400475)的碱基一致性为99.71%。次级内共生菌除Wolbachia外,Rickettsia、Cardinium、Hamlitonella均可感染桑粉虱。81份桑粉虱样本中,次级内共生菌Rickettsia、Cardinium、Hamlitonella的感染率分别为6.2%、11.1%、11.1%;Cardinium与Hamlitonella的共同感染率为11.1%,Rickettsia与Hamlitonella、Rickettsia与Cardinium、Rickettsia与Cardinium和Hamlitonella的共同感染率皆为6.2%;88.9%的样本没有检测到次级内共生菌感染。结果表明,桑粉虱体内检测到Rickettsia、Cardinium、Hamlitonella3种次级内共生菌感染,Hamlitonella、Cardinium的感染率相同并高于Rickettsia;桑粉虱体内3种次级内共生菌的感染率及共同感染率较低。研究结果可为桑粉虱内共生菌的鉴定和功能研究等提供参考。
Pealius mori was one of the most serious pests causing harm to mulberry tree in sericulture areas of China.Genomic DNA of P.mori from mulberry field was amplified and sequenced by PCR using specific primers from wsp gene of secondary endosymbiotic bacteria Wolbachia and from 16 S rDNA of secondary endosymbiotic bacteria Rickettsia,Cardinium and Hamlitonella.The type of secondary endosymbiotic bacteria was identified by analyzing the gene sequence,and infection conditions of P.mori samples were also detected and analyzed.Nucleotide similarity between amplified sequences from Rickettsia in P.mori samples(963 bp)and from Rickettsia in Bemisia tabaci samples(KY620205,GU563843,FJ915603)was 99.90% to 100%.Nucleotide similarity between amplified sequences from Cardinium in P.mori samples(446 bp)and from Cardinium in B.tabaci samples(FJ766341,GU563845,HG421084,KT827382)was 99.78%.Nucleotide similarity between amplified sequences from Hamlitonella in P.mori samples(694 bp)and from Hamlitonella in B.tabaci samples(MH908669,KM197206,AB981348,AF400475)was 99.71%.Moreover,secondary endosymbiotic bacteria of Rickettsia,Cardinium and Hamlitonella could all infect 81 P.mori samples apart from Wolbachia,whose infection rate were 6.2%,11.1% and 11.1%,respectively.Co-infection rate of Cardinium and Hamlitonella was 11.1%,and co-infection rate of Rickettsia and Hamlitonella was 6.2%,the same as that of Rickettsia and Cardinium and that of Rickettsia,Cardinium and Hamlitonella.There were 88.9% P.mori samples uninfected with secondary endosymbiotic bacteria.The results showed that 3 kinds of secondary endosymbiotic bacteria could be detected in P.mori samples,including Rickettsia,Cardinium and Hamlitonella.Besides,infection rates of Hamlitonella and Cardinium to P.mori were the same though they were both higher than that of Rickettsia,and infection rate and co-infection rate of 3 kinds of secondary endosymbiotic bacteria in P.mori were all low.The results of this study will provide a reference for identification and function study on endosymbiotic bacteria in P.mori.
作者
柴建萍
江秀均
杨振国
倪婧
罗雁婕
谢道燕
黄平
Chai Jianping;Jiang Xiujun;Yang Zhenguo;Ni Jing;Luo Yanjie;Xie Daoyan;Huang Ping(Institute of Sericulture and Apiculture,Yunnan Academy of Agricultural Sciences,Mengzi Yunnan 661101,China)
出处
《蚕业科学》
CAS
CSCD
北大核心
2019年第6期805-810,共6页
ACTA SERICOLOGICA SINICA
基金
现代农业产业技术体系建设专项(No.CARS-18)
云南省农业基础研究联合面上项目(No.2018FG001-070)。
