摘要
为建立快速多信息薄层鉴别方法,采用薄层色谱,分别以三氯甲烷-乙酸乙酯-浓氨试液(10∶1∶0.1)、环己烷-乙酸乙酯-甲酸(12∶2∶0.2)、三氯甲烷-乙酸乙酯-甲醇-浓氨试液(4∶2∶4∶0.5)和三氯甲烷-乙酸乙酯-甲醇-浓氨试液(1∶2∶4∶0.5)为展开剂,通过紫外光灯365 nm、254 nm、日光和灯光检视,共检出绵马贯众脂溶性、中极性和水溶性成分斑点18个。该方法简便、快捷、实用,可多指标、快速控制绵马贯众药材的质量,辨别其真伪。
In order to establish a rapid and multi information identification method,a TLC method was used,a mixture of trichloromethane,ethyl acetate and strong ammonia solution(10∶1∶0.1),cyclohexane,ethyl acetate and formic(12∶2∶0.2),trichloromethane,ethyl acetate,methanol and strong ammonia solution(4∶2∶4∶0.5),chloroform,ethyl acetate,methanol and concentrated ammonia(1∶2∶4∶0.5)was used as the developer solution respectively,spots of Dryopteridis Crassirhizomatis Rhizoma were viewed under UV 365 nm,UV 254 nm,the daylight and the lights respectively.A total of 18 component spots of the liposoluble,the intermediate polar and water-soluble from Dryopteridis Crassirhizomatis Rhizoma were observed.This method is simple,rapid and practical,which could control the quality of the medicinal materials quickly and identify the authenticity.
作者
赵丽丽
缪雪荣
兰新财
安丽娜
ZHAO Li-li;MIAO Xue-rong;LAN Xin-cai;AN Li-na(Zhejiang Jindakang Animal Health Co.,Ltd,Jinhua,Zhejiang 321016,China)
出处
《中国兽药杂志》
2020年第4期52-55,共4页
Chinese Journal of Veterinary Drug
关键词
绵马贯众
多信息
薄层色谱法
Dryopteridis Crassirhizomatis Rhizoma
multi-information
thin-layer chromatography
