摘要
EMS诱变辣椒亲本‘S15’获得1个耐涝突变体‘RW15’。前期研究认为,保护酶和内源激素代谢相关的8个基因与其耐涝特性相关。本研究通过对不同水涝处理时间的‘S15’和‘RW15’的根和叶进行基因实时定量分析,以探讨其在耐涝机制中的作用。研究发现,水涝胁迫下,生长素诱导蛋白相关的Cap.ARATH,乙烯响应相关的Cap.RAP2、MYB家族相关的Cap.MYB1R1和过氧化物酶Cap.POD相关的4个基因表达量等7个基因表达量在突变体中显著升高,另1个与过氧化物酶Cap.POD相关Capana02g003649基因表达量却下降;另外,同一处理时间的‘RW15’对水涝胁迫响应时间快及响应程度高于‘S15’,Cap.MYB1R1表达量最高,且胁迫前后表达差异达到6.94倍。研究表明,突变体‘RW15’主要通过提高内源激素和保护酶代谢等相关功能基因的表达达到耐涝能力的增强,该研究对突变体耐涝机制解析提供数据了参考。
A waterlogging-tolerant mutant'RW15'was obtained from pepper parent'S15'by EMS mutagenesis.Previous studies suggested that 8 genes related to protective enzymes and endogenous hormone metabolism were associated with waterlogging tolerance.In this paper,real-time quantitative genetic analysis was carried out on roots and leaves of'S15'and'RW15'with different waterlogging treatment times to explore its role in waterlogging resistance mechanism.It was found that under waterlogging stress,the expression levels of 7 genes,including Cap.ARATH related to auxin induced protein,Cap.RAP2 related to ethylene response,Cap.MYB1R1 related to MYB family and Cap.POD related to peroxidase,were significantly increased in the mutant,while the expression levels of another Cap.POD-related Capana02g003649 was decreased.At the same time,it was found that the response time of'RW15'to waterlogging stress was faster and the degree of response was higher than that of'S15'at the same treatment time,and the expression level of Cap.MYB1R1 was the highest,and the expression difference before and after stress reached 6.94 times.Studies have shown that the mutant'RW15'mainly enhances its waterlogging resistance by improving the expression of relevant functional genes such as endogenous hormones and protective enzyme metabolism,which laid a foundation for the mechanism analysis of waterlogging tolerance mechanism of mutants.
作者
谭放军
龙春花
宋静爽
欧立军
Tan Fangjun;Long Chunhua;Song Jingshuang;Ou Lijun(Vegetable Rescarch Institution,Hunan Academy of Agricultural Science,Changsha,410125;College of Biology,Hunan University,Changsha,410128;Long Ping Branch,Graduate School of Hunan University,Changsha,410125)
出处
《分子植物育种》
CAS
CSCD
北大核心
2020年第8期2460-2467,共8页
Molecular Plant Breeding
基金
湖南省自然科学基金(2017JJ2147)资助。
