摘要
Although calcium sulfate bone cements possess favorable characteristics of excellent clinical handling and osteoconductivity,the rapid degradation and poor bioactivity limit their wide applications.This study reports a type of novel injectable and self-setting composite materials for bone defect repair.Magnesium substituted tricalcium phosphate nanoparticles(43.8±9.0 nm)and calcium sulfate hemihydrate micro-size particles(5–21μm)were mixed with a polyvinyl alcohol solution with optimal compositions to obtain the composite materials,which showed reasonable initial setting time(11.7–19.2 min)and suitable compressed strength(2.28–6.33 MPa).By utilizing magnesium powder as a porogen,macro-pores(>100μm)together with micro-pores were created in the final product after setting.In addition,MC3T3-E1 cells extended well and exhibited numerous lamellipodia and long filopodia when cultured with the composite materials,indicating that they had no cytotoxicity.The in vivo results indicated that the injectable composite materials could achieve bone defect repair,when implanted in beagle femoral condyle defects for 10 months.Our results show that the new injectable composite materials are biocompatible and biodegradable,which possess great potential for bone defect repair.
硫酸钙骨水泥具有良好的骨传导性,但降解速率快、生物活性差的缺点限制了其临床应用.本文将β-磷酸三钙纳米颗粒(粒径43.8±9.0 nm)和半水硫酸钙颗粒(粒径5–21μm)混合作为固相,与液相聚乙烯醇溶液(5 wt.%)按优化重量比混匀,制备了可注射自固化复合材料.该材料具有合理的自固化时间(11.7–19.2 min)及适宜的压缩强度(2.28–6.33 MPa).同时,利用镁颗粒作为成孔剂,制备出大孔径(大于100μm)的多孔支架.体外细胞实验显示,MC3T3-E1细胞伸展良好,表现出大量的板状伪足和伸展的丝状伪足,表明该复合材料无细胞毒性.将可注射复合材料植入比格犬股骨髁缺损区,10个月后骨缺损愈合良好,表明该材料具有良好的骨缺损修复潜力.
作者
Qian Xu
Jiulong Liang
Haipeng Xue
Yun’en Liu
Lei Cao
Xiaowu Li
Jing Tian
Xing Zhang
徐倩;梁久龙;薛海鹏;柳云恩;曹磊;李小武;田竞;张兴(Department of Materials Physics and Chemistry,School of Materials Science and Engineering,Key Laboratory for Anisotropy and Texture of Materials,Ministry of Education,Northeastern University,Shenyang 110819,China;Institute of Metal Research,Chinese Academy of Sciences,Shenyang 110016,China;General Hospital of Northern Theater Command,Shenyang 110016,China;School of Materials Science and Engineering,University of Science and Technology of China,Hefei 230026,China)
基金
supported by the Key Research Program of Frontier Sciences(QYZDY-SSW-JSC031)from the Chinese Academy of Sciences(CAS)
the Key Research and Development Program of Liaoning Province(201703031)
Liaoning Science and Technology Project(20170540972)
Shenyang Key R&D and Technology Transfer Program(Z17-7-023).
