摘要
目的:研究血浆纤维凝胶蛋白-3(Ficolin-3)水平与肺炎支原体(MP)感染患儿疾病分期及病情程度的相关性。方法:选取本院2018年6月至2019年6月儿内科收治的MP感染的患儿78例作为研究对象,同时选取同期儿内科门诊健康体检儿童88例作为对照组,采用酶联免疫吸附法(ELISA)测定受试儿童血浆中Ficolin-3表达水平和血清中白细胞介素-8(IL-8)、白细胞介素-10(IL-10)、肿瘤坏死因子(TNF-α)、采用免疫散射比浊法测定血清免疫球蛋白(IgM、IgA)表达水平,分析Ficolin-3表达水平与患儿疾病分期、病情程度、IL-8、IL-10、TNF-α、IgM、IgA表达水平的相关性。结果:与对照组相比,MMP患儿组血浆中Ficolin-3、IL-8、TNF-α、IgM表达水平显著升高(P<0.05),IL-10、IgA表达水平显著降低(P<0.05);与恢复期组相比,急性期组患儿血浆中Ficolin-3、IL-8、TNF-α、IgM表达水平显著升高(P<0.05),IL-10、IgA表达水平显著降低(P<0.05);与轻微型组相比,严重型组患儿血浆中Ficolin-3、IL-8、TNF-α、IgM表达水平显著升高(P<0.05),IL-10、IgA表达水平显著降低(P<0.05);MMP患儿血浆中Ficolin-3表达水平与患儿疾病分期、病情严重程度、血清中IL-8、TNF-α、IgM表达水平呈正相关(P均<0.05),与血清中IL-10、IgA表达水平呈负相关(P<0.05)。结论:Ficolin-3在MMP患者血浆中表达水平显著升高,与患儿疾病分期、病情程度、血清IL-8、TNF-α、IgM表达水平呈正相关,与血清IL-10、IgA表达水平呈负相关,可能成为评价肺炎支原体肺炎(MMP)患儿病情的临床指标。
Objective:To study the correlation between plasma fibrin gel-3(Ficolin-3)level and disease stages,severity of children with Mycoplasma pneumoniae(MP)infection.Methods:78 children with MP infection admitted to the Department of Pediatrics from June 2018 to June 2019 were selected as the study objects,and 88 children with MP infection were selected as the control group,the expression levels of ficolin-3 in plasma,interleukin-8(IL-8),interleukin-10(IL-10),tumor necrosis factor(TNF-α)were measured by enzyme-linked immunosorbent assay(ELISA),detection of serum immunoglobulin(IgM,IGA)expression by immunoturbidimetry and the correlations between the expression level of ficolin-3 and disease stage,disease degree,IL-8,IL-10,TNF-α,IgM,IgA were analyzed.Results:Compared with the control group,the levels of ficolin-3,IL-8,TNF-αand IgM in the plasma of MMP group were significantly higher(P<0.05),while the levels of IL-10 and IgA were significantly lower(P<0.05).Compared with the convalescent group,the levels of ficolin-3,IL-8,TNF-αand IgM in the plasma of the acute group were significantly higher(P<0.05),while the levels of IL-10 and IgA decreased significantly(P<0.05).Compared with the mild group,the expression levels of ficolin-3,IL-8,TNF-αand IgM in the severe group were significantly higher(P<0.05),while the expression levels of IL-10 and IgA were significantly lower(P<0.05).The expression level of ficolin-3 in plasma of children with MMP was positively correlated with disease stage,disease severity,the expression levels of IL-8,TNF-αand IgM in serum(P<0.05),and negatively correlated with the expression levels of IL-10 and IgA in serum(P<0.05).Conclusion:The expression level of ficolin-3 in plasma of MMP patients is significantly increased,which is positively correlated with disease stage,disease degree,expression levels of serum IL-8,TNF-αand IgM,and negatively correlated with the expressions of serum IL-10 and IgA,it may be a clinical index to evaluate the condition of children with Mycoplasma pneumoniae pneumonia(MMP).
作者
曹燕玲
高娇娇
李文斌
李春梅
刘振奎
CAO Yanling;GAO Jiaojiao;LI Wenbin(The First Affiliated Hospital of Hebei North University, Hebei Zhangjiakou 075000, China)
出处
《河北医学》
CAS
2020年第4期693-697,共5页
Hebei Medicine
基金
河北省医学科学研究重点课题计划项目,(编号:20181310)。
