摘要
天然虾青素具有突出的生物活性和极高的经济价值。然而,许多研究仍然以丙酮等有机试剂提取虾青素,不仅给环境带来巨大压力,而且降低了生产安全性。为探索提取天然虾青素的高效绿色工艺,构建一种新型离子液体-盐双水相体系,考察离子液体种类、温度、pH值等因素对该体系相行为以及虾青素分配系数的影响规律,并从雨生红球藻中提取天然虾青素。结果表明,辛基三丁基溴化膦([P4448]Br)、辛基三丁基氯化膦([P4448]Cl)、四丁基溴化膦([P4444]Br)、四丁基氯化膦([P4444]Cl)、四丁基三氟乙酸膦([P4444]CF3COO)以及四丁基溴化铵([N4444]Br)可与K2HPO4/KH2PO4形成具有低临界共熔温度(LCST)的离子液体-盐双水相体系,其中[P4448]Br形成双水相的能力及其双水相体系萃取虾青素的能力均为最强。在超声功率75 W、超声时间60 min的条件下,[P4448]Br-K2HPO4/KH2PO4双水相体系从雨生红球藻中获得虾青素的萃取率达96.09%。研究结果从新的角度审视双水相体系相行为和生物分子迁移特性之间的关联,为采用双水相分离技术从生物资源中提取天然虾青素提供了理论依据。
Natural astaxanthin has outstanding biological activity and high economic value.However,many studies are still limited to the astaxanthin extraction with organic solvents such as acetone,which causes great pressure on the environment,and reduces the production safety.To explore an efficient and green process for astaxanthin extraction,novel ionic liquid(IL)-based aqueous biphasic systems(ABSs)were constructed.The effects of IL,temperature,pH on the phase behavior of the ABSs and astaxanthin distribution were investigated.The results showed that tributyloctylphosphonium bromide([P4448]Br),tributyloctylphosphonium chlorine([P4448]Cl),tetrabutylphosphonium bromide([P4444]Br),tetrabutylphosphonium chloride[P4444]Cl,tetrabutylphosphonium trifluoroacetate([P4444]CF3COO),and tetrabutylammonium bromide([N4444]Br)could form low critical solution temperature(LCST)-type ABSs with K2HPO4/KH2PO4.[P4448]Br has the strongest ability to form ABS and the[P4448]Br-based ABS showed the highest extraction yield of astaxanthin.When the ultrasonic power of 75 W and ultrasonic time of 60 min,the extraction yield of astaxanthin in obtained from Haematococcus pluvialis by[P4448]Br-K2HPO4/KH2PO4 ABS was 96.09%.The results help us to understand the correlation between the phase behavior of the ABS and the extraction of biomolecule,and provide an important theoretical basis for the astaxanthin extraction from bioresources.
作者
张莉莉
高静
魏媛仪
聂芳红
吉宏武
刘书成
毛伟杰
Zhang Lili;Gao Jing;Wei Yuanyi;Nie Fanghong;Ji Hongwu;Liu Shucheng;Mao Weijie(College of Food Science and Technology,Guangdong Ocean University,Zhanjiang 524088,Guangdong;Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution,Zhanjiang 524088,Guangdong;Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety,Zhanjiang 524088,Guangdong)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2020年第4期170-178,共9页
Journal of Chinese Institute Of Food Science and Technology
基金
国家自然科学基金青年科学基金项目(21706040)
广东省自然科学基金面上项目(2019A1515010640)
广东省创新强校特色创新项目(230419064)
广东省“攀登计划”专项(pdjh2020b0276)。
关键词
虾青素
雨生红球藻
离子液体
双水相
萃取
astaxanthin
Haematococcus pluvialis
ionic liquid
aqueous biphasic system
extraction
