摘要
目的建立一种高效液相色谱-串联质谱(HPLC MS/MS)同时测定五味子糖浆中7种木脂素成分含量的方法。方法采用Agilent ZORBAX Eclipse XDB C1(84.6 mm×150 mm,5μm)色谱柱,以90%甲醇(含0.1%甲酸) 水(含0.1%甲酸,5 mmol乙酸铵)为流动相,流速0.5 mL/min,柱温30℃,进样量:10μL;采用电喷雾电离源(ESI),正离子扫描,多反应离子监测模式(MRM)进行定量分析。结果五味子糖浆中7种木脂素成分分离度良好,在测定范围内线性关系均良好(r>0.995),平均回收率分别为98.66%,92.41%,95.15%,100.46%,96.67%,100.94%,98.83%。结论该方法快速、灵敏、可靠,可满足五味子糖浆中五味子醇甲、五味子醇乙、五味子甲素、五味子乙素、五味子丙素、五味子酚和五味子酯甲的含量测定。
Objective To establish a method for simultaneous determination of seven lignan components in Wuweizi syrup by HPLC MS/MS.Methods The chromatographic separation was performed on a Agilent ZORBAX Eclipse XDB C18(4.6 mm×150 mm,5μm)column with the mobile phase consisting 90%methanol(containing 0.1%formic acid) water(containing 0.1%formic acid,5 mmol ammonium acetate)at a flow rate of 0.5 mL/min,the column temperature was 30℃and the injection volume was 10μL.An electrospray positive ion multiple reaction monitoring(MRM)was used as a detector.Results The seven lignan components in Wuweizi syrup had good separation and good linearity in the determination range(r>0995).The average recoveries were 98.66%,92.41%,95.15%,100.46%,96.67%,100.94%and 98.83%,respectively.Conclusion This method is rapid,sensitive and reliable,which can be used to determine the content of schisandrin,schisandrol B,deoxyschizandrin,schizandrin B,schizandrin C,schisanhe nol and schisantherin A in Wuweizi syrup.
作者
吴子静
梁大虎
杨斌
谢海棠
WU Zijing;LIANG Dahu;YANG Bin;XIE Haitang(Wannan Medical College,Wuhu,Anhui 241002,China;Yijishan Hospital,The First Affiliated Hospital of Wannan Medical College,Anhui Provincial Centre for Drug Clinical Evaluation,Wuhu,Anhui 241001,China)
出处
《安徽医药》
CAS
2020年第5期869-873,共5页
Anhui Medical and Pharmaceutical Journal
基金
皖南医学院中青年科研基金(WK2018F05)。
关键词
木脂素类
色谱法
高压液相
串联质谱法
五味子糖浆
液相
质谱联用
含量测定
Lignans
Chromatography
high pressure liquid
Tandem mass spectrometry
Wuweizi syrup
HPLC MS/MS
Content determination
