摘要
应用太赫兹时域光谱技术结合移动窗口相关系数法筛选与种子老化密切相关的太赫兹特征波段。选取3个不同品种的玉米种子,并制备人工老化0、1、2、3、4 d的实验样本,采用衰减全反射(Attenuated total reflection,ATR)附件采集从样本分离得到的种胚和胚乳粉末的太赫兹吸光度谱(0.2~80 cm^-1),应用移动窗口相关系数法(窗口宽度为10,阈值为0.3)筛选样本种胚和胚乳特征谱区。结果表明,不同品种样本筛选得到的老化特征谱区差异显著,说明种子老化进程受品种影响较大;在相同的老化阶段,不同品种样本的特征谱区也存在部分共同区间,且主要集中在60~80 cm^-1,说明老化进程中有些成分的变化相近;种胚和胚乳在相同老化阶段筛选的特征谱区不同,说明两者在老化进程中生理变化存在差异。本研究表明太赫兹时域光谱结合化学计量学方法可用于快速表征并探测玉米种子老化的动态变化过程。
Characteristic Terahertz( THz) region closely related to corn seed aging were screened by using THz time domain spectroscopy technique and moving window correlation coefficient method. There were three different varieties of corn seeds selected and processed by artificial aging for 0 d,1 d,2 d,3 d and 4 d. The embryos and endosperms of corn seeds samples were isolated and pulverized. THz absorbance spectra( 0. 2 ~ 80 cm^-1) of the powder samples were collected by THz time domain system and attenuated total reflection( ATR) accessory. The moving window correlation coefficient method was applied to screen the characteristic spectral regions which were sensitive to seed aging. After comparing the parameters of the moving window correlation coefficient,the window width was determined to 10 and threshold value was 0. 3. According to the result,the selected characteristic spectral regions showed significant differences among different varieties of samples,which indicated that the seed aging process was greatly affected by the variety. However,there were minor common regions in the same aging stage which were mainly concentrated in 60 ~ 80 cm^-1. It was showed that the similar components were changed at the same time. Due to the selected characteristic spectra of endosperm and embryo at the same aging stage were different,it was showed that the endosperm and embryo had different physiological changes during seed aging process. The experiment result indicated that THz time domain spectroscopy combined with chemometric method can be used to characterize and detect the dynamic changes of corn seed aging rapidly and macroscopically.
作者
吴静珠
李晓琪
刘翠玲
孙晓荣
余乐
孙丽娟
WU Jingzhu;LI Xiaoqi;LIU Cuiling;SUN Xiaorong;YU Le;SUN Lijuan(Beijing Key Laboratory of Big Data Technology for Food Safety,Beijing Technology and Business University,Beijing 100048,China;Institute of Crop Science,Chinese Academy of Agricultural Sciences,Beijing 100081,China)
出处
《农业机械学报》
EI
CAS
CSCD
北大核心
2020年第4期382-386,393,共6页
Transactions of the Chinese Society for Agricultural Machinery
基金
国家自然科学基金项目(61807001)
国家留学基金委访学项目(2019)
北京工商大学基本科研业务费项目(PXM2019_014213_000007)。
关键词
种子活力
太赫兹时域光谱
特征波段
衰减全反射
移动窗口相关系数法
seed vigor
Terahertz time domain spectroscopy
characteristic region
attenuated total reflection
moving window correlation coefficient method
