摘要
利用Illumina高通量测序对绿球藻(Chlorococcum sp.)GIEC-38的转录组进行测定,分析在氮(N)胁迫(无氮源BG-11)下产油高的藻株与原始培养条件相比光合固碳富集油脂相关的代谢通路和基因表达情况,发现在N胁迫下藻内有14817个基因表达上调、15282个基因表达下调。光合作用、叶绿素代谢和氮代谢等代谢通路均有明显变化,代谢途径可能受到一定的抑制进而增加油脂合成途径。对藻株TAG代谢重建后发现乙酰辅酶A羧化酶等许多与脂类合成相关的基因表达量显著上调,分布在TAG合成中12个步骤中,这些均可促进脂质的合成,提高细胞的油脂产量。
The transcriptome and gene expression of Chlorococcum sp. GIEC-38 were sequenced by an Illumina sequencing platform-based RNA-Seq approach. The metabolic pathway and gene expression related to photosynthetic carbonsequestration and lipid enrichment of an original algal strain cultured in BG-11 and BG-11 without nitrogen were analyzed.It was found that 14817 reads of genetic expression were up-regulated and 15282 reads of genetic expression were downregulated in algae cells under nitrogen deficiency stress. The gene expression levels of metabolic pathways such as photosynthesis,chlorophyll metabolism,and nitrogen metabolism had obvious changed. Gene expression including AcetylCoA carboxylase and other important genes involved in 12 steps in lipid synthesis pathway were significantly up-regulated in Chlorococcum sp. GIEC-38 under nitrogen deficiency stress after reconstructing TAG biosynthesis pathway. All these changes promoted the synthesis of lipids and increased the lipid productivity of GIEC-38 cells.
作者
冯佳
朱顺妮
许瑾
王忠铭
袁振宏
Feng Jia;Zhu Shunni;Xu Jin;Wang Zhongming;Yuan Zhenhong(Guangzhou Institute of Energy Conversion,Chinese Academy of Sciences,Key Laboratory of Renewable Energy,Chinese Academy of Sciences,Guangdong Key Laboratory of New and Renewable Energy Research and Development,Guangzhou 510640,China;Collaborative Innovation Centre of Biomass Energy,Zhengzhou 450002,China)
出处
《太阳能学报》
EI
CAS
CSCD
北大核心
2020年第2期13-19,共7页
Acta Energiae Solaris Sinica
基金
国家重点研发计划(2016YFB0601004)
广东省自然科学基金(2016A030312007)
广东省自然科学基金(2015A030310032)
广东省自然科学基金(2016A030313171)。
关键词
光合作用
油脂
基因表达
微藻
上调
photosynthesis
lipids
gene expression
microalgae
up-regulated
