摘要
2019新型冠状病毒(SARS-CoV-2)是一种在人体中新发现的冠状病毒,来源于野生动物。SARS-CoV-2属于冠状病毒科β冠状病毒属,而感染宠物犬猫类的冠状病毒主要来自α冠状病毒属。SARS-CoV-2是否感染犬、猫等宠物是当前抗击新冠肺炎疫情的重要公共卫生问题,也是大众关切的热点问题之一。本研究试图通过对有明晰腹泻和呼吸道症状的宠物犬和猫进行病原学检测,来阐明SARS-CoV-2是否感染宠物的科学问题,探究腹泻和呼吸症状宠物感染的普遍病因。本研究采用SARS-CoV-2的荧光定量PCR试剂和已经建立的诊断方法,对SARSCoV-2流行期间北京地区有腹泻和呼吸道症状的(特别是有发烧、严重咳嗽症状)临床宠物猫病例(20例)和宠物犬病例(4例)采集咽拭子,并进行SARS-CoV-2核酸荧光定量PCR检测和犬瘟热病毒(Canine distemper virus,CDV)、犬细小病毒(Canine parvovirus,CPV)、犬腺病毒Ⅱ型(Canine adenoviru-2,CAV-2)、犬副流感病毒(Canine parainfluenza virus,CPIV)、猫疱疹病毒I型(Feline herpesvirus-1,FHV-1)及猫杯状病毒(Feline calicivirus,FCV)、支原体(Mycoplasma,MY)及衣原体(Chlamydophila,CPS)等病原的检测。结果表明,所有样本SARS-CoV-2均为阴性,但分别存在其它病原感染。
2019 novel coronavirus(SARS-CoV-2)is a new strain of coronavirus that has never been found in humans. SARS-CoV-2 is a β coronavirus,whereas the coronaviruses infecting pet dogs and cats arise mainly from α-coronaviruses. Whether SARS-CoV-2 infects cats,dogs and other pets is an important public-health issue during this time. In the present study,respiratory-tract symptoms in 20 pet cats and 4 pet dogs(especially with obvious fever and cough symptoms) in Beijing,China,were detected by fluorescence quantitative polymerase chain reaction(PCR) of SARS-CoV-2 and established diagnostic methods. Throat swabs were collected to detect the nucleic acids of SARS-CoV-2 using fluorescence quantitative PCR and to detect other pathogens. The nucleic acids of SARS-CoV-2 were not present in the 24 pets that we evaluated.
作者
崔尚金
赵花芬
纪志辉
石文达
熊国英
梁琳
陈海博
王祉国
刘坡
韩武
张伟
王宏伟
CUI Shangjin;ZHAO Huafen;JI Zhihui;SHI Wenda;XIONG Guoying;LIANG Lin;CHEN Haibo;WANG Zhiguo;LIU Po;HAN Wu;ZHANG Wei;WANG Hongwei(Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193,China;ZHONGRUI(Beijing)Pet's Disease Test Biotechnology Co.,Ltd.,Beijing 102629,China;Najiya Beibei Pet's Hospital,Beijing 100192,China;Najiya Yabo Pet's Hospital,Beijing 100191,China;Najiya Tongzhou Pet's Hospital,Beijing 100101,China;Aichongkang Pet's Hospital,Beijing 102399,China;Kangkang Pet's Hospital,Beijing 101312,China)
出处
《病毒学报》
CAS
CSCD
北大核心
2020年第2期170-175,共6页
Chinese Journal of Virology
