摘要
叶色突变体是研究光合作用及叶绿体发育的重要材料。开展玉米叶色突变体的相关研究,对光形态建成、光合作用、基因功能注释、蛋白质功能及抗逆性机制的阐述具有重要的理论意义。本研究以黄绿叶突变体ygl-F17138为材料,与玉米自交系B73进行杂交,构建F2分离群体,进行遗传效应分析和基因初步定位。遗传分析表明,该突变性状由单个隐性核基因控制,且能稳定遗传。利用BSR-seq结合连锁分析的方法将该基因初步定位在第3条染色体上一个约9.2 Mb的区间内(chr.3:173087201~182203992),查询该区间内已知基因功能注释,未发现类似前人报道的调控黄绿叶性状基因,说明YGL-F17138基因可能是一个控制玉米黄绿叶发育未被挖掘的候选基因。
Leaf-color mutants are important materials for deciphering the photosynthesis and chloroplast development.The research on maize leaf color mutants has important theoretical significance for the description of photomorphogenesis,photosynthesis,gene function annotation,protein function,metabolic pathway and stress resistance mechanism.In this study,a yellow-green leaf mutant was identified and designated ygl-F17138.By making a cross with the maize inbred line B73,we constructed a F2 segregation population for the genetic analysis and the preliminary gene mapping.Genetic analysis indicated that this yellow-green phenotype was genetically controlled by a recessive nuclear gene.By deployment of BSR-seq and the linkage analysis method,this gene was allocated to an interval of about 9.2 Mb on chromosome 3(chr.3:173087201-182203992).According to the functional annotation of known genes in this interval,no genes which regulating yellow green leaf traits similar to those previously reported were found,indicating that YGL-F17138 gene may be an untapped candidate gene for controlling the development of yellow green leaf in maize.
作者
刘忠祥
寇思荣
连晓荣
杨彦忠
王晓娟
何海军
周玉乾
周文期
LIU Zhong-xiang;KOU Si-rong;LIAN Xiao-rong;YANG Yan-zhong;WANG Xiao-juan;HE Hai-jun;ZHOU Yu-qian;ZHOU Wen-qi(Crops Research Institute,Gansu Academy of Agricultural Sciences,Lanzhou 730070)
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2020年第2期452-458,共7页
Journal of Plant Genetic Resources
基金
国家自然科学基金项目(31760390,31860384)
国家重点研发计划(2018YFD0100202-3,2016YFD0100103-19)。
