摘要
目的探讨膈下逐瘀汤加减方对气滞血瘀型慢性输卵管炎大鼠中医证候积分、血液流变学及肿瘤坏死因子-α(TNF-α)、干扰素β(IFN-β)的影响。方法将60只SD雌性大鼠随机分为正常组、复合模型组、膈下逐瘀汤高剂量组、膈下逐瘀汤中剂量组、膈下逐瘀汤低剂量组,每组12只。除正常组大鼠外,余组大鼠先于输卵管接种金黄色葡萄球菌制备成输卵管炎症模型,7 d后进行声、光、电+夹尾复合刺激21 d造气滞血瘀模型。从造模第29天开始,膈下逐瘀汤高、中、低剂量组分别给予相应浓度的膈下逐瘀汤加减方灌胃,复合模型组给予等体积蒸馏水灌胃,持续28 d。于末次灌胃后的第2天对各组大鼠进行中医证候评分,行腹主动脉取血,测定各组大鼠血液流变学及血清TNF-α、IFN-β水平。结果复合模型组大鼠的中医证候积分、血浆黏度、还原黏度3.0、全血黏度30及血清TNF-α、IFN-β水平均明显高于正常组(P均<0.05)。膈下逐瘀汤高、中剂量组中医证候积分、血浆黏度、还原黏度3.0、全血黏度30及血清TNF-α、IFN-β水平均明显低于复合模型组(P均<0.05),膈下逐瘀汤高、中剂量组间各指标比较差异均无统计学意义(P均>0.05)。结论膈下逐瘀汤加减方可以明显改善慢性输卵管炎大鼠气滞血瘀证候,降低血液黏度,其可能通过降低TNF-α、IFN-β的表达水平而发挥免疫调节及抗炎作用。
Objective It is to observe the effect of Gexia Zhuyu Decoction on the scores of traditional Chinese medical syndrome,hemorheological indexes and tumor necrosis factor-α(TNF-α)and interferonβ(IFN-β)in rats with chronic salpingitis with Qi-stagnancy and blood stasis.Methods Sixty female SD rats were randomly divided into normal group,compound model group,Gexia Zhuyu Decoction high dose group,Gexia Zhuyu decoction middle dose group,Gexia Zhuyu decoction low dose group,12 rats in each group.The rats in all the groups except for the normal group were vaccinated with staphylococcus aureus in the fallopian tube to prepare the model of salpingitis.In 7 days after modeling,the model of qi-stagnancy and blood stasis was prepared by compound stimulation of sound,light and electric plus clip tail for 21 days.From the 29th day of model making,the Gexia Zhuyu Decoction high,middle and low dose groups were given the corresponding concentration of the decoction by gavage,and the compound model group was given equal volume distilled water,all the groups were treated for 28 d.At the 2nd day after the last gavage,the scores of traditional Chinese medical syndrome of the rats in each group were assessed,their blood was collected from abdominal aorta to detect the levels of hemorheology and serum TNF-α、IFN-β.Results The scores of traditional Chinese medical syndrome,plasma viscosity,reduced viscosity 3.0,whole blood viscosity 30 and the levels of serum TNF-α、IFN-βin the rats of compound model group were significantly higher than those in the normal group(P<0.05).The scores of traditional Chinese medical syndrome,plasma viscosity,reduced viscosity 3.0,whole blood viscosity 30 and the levels of serum TNF-α、IFN-βin the rats of the high and middle dose groups were significantly higher than those in the compound model group(P<0.05).There was no significant difference in the above indexes between the Gexia Zhuyu Decoction high dose group and Gexia Zhuyu Decoction middle dose group(P>0.05).Conclusion Gexia Zhuyu Decoction can obviously improve the syndrome of qi-stagnancy and blood stasis and reduce blood viscosity in the rats with chronic salpingitis,which may play an immunomodulatory and anti-inflammatory role by reducing the expression level of TNF-α,IFN-β.
作者
刘丽
程英龙
刘进哲
王亚文
郑跃
LIU Li;CHENG Yinglong;LIU Jinzhe;WANG Yawen;ZHENG Yue(The First Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine, Harbin 150036, Heilongjiang,China;Heilongjiang University of Traditional Chinese Medicine, Harbin 150036, Heilongjiang,China)
出处
《现代中西医结合杂志》
CAS
2020年第11期1141-1144,1148,共5页
Modern Journal of Integrated Traditional Chinese and Western Medicine
基金
黑龙江省自然科学基金面上项目(H2015026)。
