摘要
为研究生物修复剂TF3(0.5 g·kg^-1肠杆菌干菌体+20 g·kg^-1生物炭+20 g·kg^-1有机肥)对铅污染土壤的修复效果,选取5种铅浓度土壤为研究对象,采用盆栽实验方法,研究施用修复剂TF3后,铅在油菜体内的富集、转运,以及土壤有效态铅、酶活性、微生物的响应。结果表明:TF3能够促进油菜生长、显著降低油菜地上部、地下部铅含量。与CK组相比,TF3组油菜地上部、地下部及总富集系数分别降低20.12%-71.91%、37.75%-60.13%、22.45%-68.77%。TF3组显著降低土壤有效态铅含量,降低23.10%-39.84%。添加TF3,土壤四种酶活性和微生物数量都有不同程度的增加,真菌、放线菌、细菌分别较CK组增加11.76%-40.00%、6.45%-25.61%、120.20%-290.24%。研究表明,TF3降低了污染土壤铅对油菜的胁迫作用,可以作为修复铅污染的一种有效生物修复剂。
In order to study the repairing effect of bio-remediation agent TF3(0.5 g·kg^-1 enterobacteriaceae and 20 g·kg^-1 bio-char and 20 g·kg^-1 organic fertilizer)on lead-contaminated soil,five lead concentrations were selected.The pot experiment method was used to study the enrichment and transport of lead in rapeseed and the changes of soil available lead,the enzyme activity and microbial response after application of repair agent TF3.The results showed that TF3 could promote the growth of rapeseed and significantly reduce the lead content in the above ground and under-ground parts of rapeseed.Compared with CK group,the above ground,under-ground and total enrichment factors of TF3 group were decreased by 20.12%to 71.91%,37.75%to 60.13%and 22.45%to 68.77%,respectively.The TF3 group significantly reduced the effective lead concentration in the soil by 23.10%to 39.84%.With the addition of TF3,the four enzyme activities and the number of microorganisms in the soil were increased to different degrees.The fungi,actinomycetes and bacteria were increased by 11.76%to 40.00%,6.45%to 25.61%and 120.20%to 290.24%,respectively.Our results indicated that TF3 reduced the stress of lead-contaminated soil on rapeseed and could be used as an effective bio-remediation agent for controlling lead pollution.
作者
郜雅静
李建华
卢晋晶
靳东升
郜春花
GAO Yajing;LI Jianhua;LU Jinjing;JIN Dongsheng;GAO Chunhua(College of Biological Engineering,Shanxi University,Taiyuan 030006,China;Institute of Agricultural Environment&Resources,Shanxi Academy of Agricultural Sciences,Taiyuan 030031,China)
出处
《生态科学》
CSCD
2020年第2期114-123,共10页
Ecological Science
基金
国家重点联合基金项目“复垦土壤功能微生物演变特征及定向调控”(U1710255)
山西省农科院农业科技创新研究课题“矿区复垦土壤功能微生物定向调控技术研究”(YCX2018DZYS02)
山西省科技成果转化引导专项项目“矿区复垦土壤快速培肥技术示范推广”(201804D131049)。
关键词
生物修复剂
铅含量
污染土壤
油菜
富集系数
bio-remediation agent
lead content
contaminated soil
rape
enrichment factor
