摘要
通过测定不同温度、pH对卵孵化的影响以及不同温度下桑树根结线虫卵的蛰伏特性,观察胚胎发育过程,结果显示桑树根结线虫(Meloidogyne sp.)卵孵化的最适温度是25~28℃,10℃以下发育停止,35℃以上致卵死亡;在pH值为5.0和7.0时,卵的孵化率较高,分别为46.67%和50%,pH值小于4.0或大于9.0时,卵囊内的卵存活率为0;在25℃下,卵的存活率最高,为74.31%,在10、15、20、30和35℃下存活率分别为10.24%、32.78%、53.19%、70.26%和19.21%;卵的蛰伏率在10℃时最高,为72.42%,在20、25和30℃下的蛰伏率分别为43.69%、21.83%和25.58%;在25℃下,从单胞卵发育到二龄幼虫需要的时间为10~13 d.
To study the biological characteristics and embryo development of mulberry root-knot nematode by measuring the effect of different temperature and pH value on the eggs hatching and dormancy,and observing embryonic development process,the results showed that the optimum temperature for egg hatching was 25~28℃.The egg cannot develop into larvae below 10℃,and die at 35℃or above;The egg hatching rate was higher at pH value of 5 and 7,being 46.67%and highest at 25℃,up to 74.31%,lowest at 10 down to 10.24%,eggs died below pH 4 or above pH 9;the survival rate of eggs was the 32.78%,53.19%,74.31%,70.26%,and 19.21%,respectively.The egg crouching rate was the highest at 10℃,being 72.42%,lower at 20,25 and 30℃,getting to 43.69%、21.83%and 25.58%,respectively.The time from the development of the unicellular egg to the second instar larvae was 10~13 d under 25℃.
作者
邵蝴蝶
章攀
游春平
冯岩
唐翠明
SHAO Hudie;ZHANG Pan;YOU Chunping;FENG Yan;TANG Cuiming(College of Agriculture and Biology,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China;Sericultural and Agri-food research institute,Guangdong academy of agricultural science,Guangzhou 510610,China)
出处
《仲恺农业工程学院学报》
CAS
2020年第1期6-10,共5页
Journal of Zhongkai University of Agriculture and Engineering
基金
广东省现代农业产业技术体系创新团队(2016LM1134)
广东省科技计划(2016A040402036)资助项目.
