摘要
本试验以PAR(光合有效辐射)800μmol·m^-2·s^-1,温度25℃为对照(CK),设置6个处理[L1T1(PAR 200μmol·m^-2·s^-1,4℃)、L1T2(PAR 200μmol·m^-2·s^-1,6℃)、L1T3(PAR 200μmol·m^-2·s^-1,8℃)、L2T1(PAR 400μmol·m^-2·s^-1,4℃)、L2T2(PAR 400μmol·m^-2·s^-1,6℃)和L2T3(PAR 400μmol·m^-2·s^-1,8℃)],分别处理6、12、24、48和72 h,以研究低温弱光双重胁迫对番茄苗期干物质分配以及不同器官的可溶性糖、可溶性蛋白和游离氨基酸含量的影响.结果表明:低温弱光双重胁迫使地下部分干物质分配比例减小,而对地上部分干物质分配比例无显著影响,地下部分的干物质分配比例随时间的变化与地上部分相反;低温弱光胁迫显著降低了番茄茎和叶片的可溶性糖、可溶性蛋白和游离氨基酸的含量;根的可溶性糖含量随胁迫时间的变化趋势与地上部分不一致,但根、茎、叶片的可溶性糖含量均以L2T3处理72 h含量最高,分别为94.88、77.09和41.62 mg·g^-1;根的可溶性蛋白含量随胁迫时间的变化趋势与地上部分不一致,茎和叶片的可溶性蛋白含量均以L2T3处理12 h最高,以L1T1处理72 h最低;不同器官的游离氨基酸含量随胁迫时间的变化趋势与可溶性蛋白相反;弱光对番茄干物质和营养物质含量的影响小于低温.研究证实苗期番茄在低温弱光胁迫前期,干物质和营养物质先向地上部分分配,胁迫24 h后则更多地向根系积累.
In this study,we examined the influence of low temperature and low light intensity stress on the soluble sugar,soluble protein,and free amino acid content in different organs and on dry matter distribution in greenhouse tomato seedlings.We used Photosynthetically Active Radiation(PAR)at 800μmol·m^-2·s^-1 and temperature of 25℃as control(CK),and set up an experiment involving six treatments[L1T1(PAR 200μmol·m^-2·s^-1,4℃),L1T2(PAR 200μmol·m^-2·s^-1,6℃),L1T3(PAR 200μmol·m^-2·s^-1,8℃),L2T1(PAR 400μmol·m^-2·s^-1,4℃),L2T2(PAR 400μmol·m^-2·s^-1,6℃),and L2T3(PAR 400μmol·m^-2·s^-1,8℃)]that were monitored for 6,12,24,48,and 72 h,respectively.The results showed that the dry matter distribution ratio in the underground part of the plant was lower than that in the ground-level parts and opposite to that in the aboveground part.Low temperature and low light intensity stress significantly reduced the soluble sugar,soluble protein,and free amino acid content of the stems and leaves.The soluble sugar content in the roots was not consistent with the aboveground parts but was highest in the roots,stems,and leaves(94.88 mg·g^-1,77.09 mg·g^-1 and 41.62 mg·g^-1,respectively)after 72 h in the L2T3 treatment.The soluble protein content in the roots was not consistent with the aboveground part but was highest in both the stems and leaves after 12 h in the L2T3 treatment and lowest after 72 h in the L1T1 treatment.The free amino acid content in different organs showed an effect opposite to that of the soluble protein content when the stress exposure time was prolonged.Low light had less of an effect on dry matter and nutrient content than low temperatures did.The results showed that dry matter and nutrients were distributed to the ground-level part of the plant during the early stages of exposure to stress,and then to the root system after 24 h of stress exposure.The results provide a theoretical basis for improved loss prevention due to low temperature or low light stress in crops grown in facilities such as greenhouses.
作者
杨再强
袁昌洪
丁宇晖
侯梦媛
张曼义
YANG Zaiqiang;YUAN Changhong;DING Yuhui;HOU Mengyuan;ZHANG Manyi(Collaborative Innovation Center on Forecast and Evaluation of Meteorological Disasters,Nanjing University of Information Science and Technology,Nanjing 210044;Jiangsu Key Laboratory of Agricultural Meteorology,Nanjing 210044)
出处
《南京信息工程大学学报（自然科学版）》
CAS
2020年第1期108-117,I0032-I0034,共13页
Journal of Nanjing University of Information Science & Technology（Natural Science Edition）
基金
国家重点研发计划(2019YFD1002202)
国家自然科学基金(41475107)
“十二五”国家科技支撑计划(2014BAD10B07)。
关键词
低温弱光
干物质
可溶性糖
蛋白质
氨基酸
low temperature or low light stress
dry matter
soluble sugar
protein
amino acid
