摘要
目的通过Micro-CT及三维重建技术观察天然水蛭素对大鼠缺血皮瓣血管生成的影响。方法选取32只成年SD大鼠,在其背部制备一8.0cm×1.8cm大小的缺血皮瓣模型,然后随机分为水蛭素组和对照组(n=16)。水蛭素组术后即刻和术后3 d内每天皮下注射天然水蛭素0.3 mL(含天然水蛭素6 ATU),对照组皮下注射等量生理盐水。术后6 d,大体观察皮瓣成活情况并测定皮瓣成活率,取材行HE染色观察皮瓣组织学变化,行Micro-CT三维重建观察并测量皮瓣血管容积、长度及数量。结果术后两组大鼠均存活至实验完成,无感染发生。术后6 d,两组皮瓣远端均发生不同程度坏死,水蛭素组皮瓣成活率为72.11%±8.97%,显著高于对照组的58.94%±4.02%,差异有统计学意义(t=3.280,P=0.008)。组织学观察示水蛭素组较对照组组织结构层次更清楚,有较多微血管生成,炎症反应及炎症细胞浸润更轻。Micro-CT三维重建示水蛭素组皮瓣血管更多、更加密集;血管容积、长度及数量均显著大于对照组(P<0.05)。结论天然水蛭素可减轻组织炎症反应、促进缺血皮瓣血管的新生与再通,从而提高皮瓣成活率。
Objective To investigate the effect of natural hirudin on revascularization of ischemic skin flap in rats using Micro-CT and three-dimensional(3 D)reconstruction.Methods Thirty-two Sprague Dawley rats were prepared a ischemic skin flap(8.0 cm×1.8 cm)model on the back and randomly divided into hirudin group and control group(16 rats in each group).At immediate and within 3 days after operation,the rats were treated with hypodermic injection of natural hirudin 0.3 mL(including natural hirudin 6 ATU)every day in hirudin group and the equal amount of normal saline in control group.At 6 days after operation,the survival rate of skin flap was evaluated,histological changes were observed by HE staining,and the volemia,length of blood vessels,and number of blood vessels were analyzed with MicroCT 3 D reconstruction.Results Both groups of rats survived to the end of the experiment without infection.Different degrees of necrosis occurred in the distal part of the skin flaps in both groups at 6 days after operation,but the flap survival rate of the hirudin group(72.11%±8.97%)was significantly higher than that of control group(58.94%±4.02%)(t=3.280,P=0.008).Histological observation showed that the histological hierarchy of the hirudin group was clearer than that of the control group,with more microangiogenesis and less inflammatory response and inflammatory cell infiltration.MicroCT 3 D reconstruction showed that the flap vessels in the hirudin group were more and denser,and the volemia,length of blood vessels,and number of blood vessels were significantly higher than those in the control group(P<0.05).Conclusion Natural hirudin can reduce the inflammation of tissue,promote the regeneration and recanalization of blood vessels in ischemic skin flap,so as to improve the survival rate of the flap.
作者
严玉勇
潘新元
林博杰
林冠宇
殷国前
YAN Yuyong;PAN Xinyuan;LIN Bojie;LIN Guanyu;YIN Guoqian(Department of Plastic and Aesthetic Surgery,the First Affiliated Hospital ofGuangxi M edical University,Nanning Guangxi,530021,P.R.China;Department of Cosmetology and Plastic Surgery Center,the People's Hospital ofGuangxi Zhuang Autonomous Region,Nanning Guatigxi,530021,P.R.China;Research Center for Regenerative Medicine,Guangxi Medical University,Nanning Guangxi,530021,P.R.China)
出处
《中国修复重建外科杂志》
CAS
CSCD
北大核心
2020年第3期382-386,共5页
Chinese Journal of Reparative and Reconstructive Surgery
基金
国家自然科学基金资助项目(81460297)。
