摘要
为揭示产腹泻性贝类毒素(diarrheic shellfish poisoning,DSP)典型赤潮甲藻-利玛原甲藻(Prorocentrum lima)PL11的共附生菌群多样性信息,通过Illumina MiSeq高通量测序分析了PL11的共附生菌群种类及相对丰度,并且对其可培养共附生菌株进行了选择性分离及其16S rRNA基因序列扩增与系统发育的分析。利玛原甲藻PL11高通量测序结果显示:样品共附生细菌包括5门,14纲,26目,38科及54属。其中优势门(>5%)为3个,包括变形菌门(Proteobacteria,75.5%)、拟杆菌门(Bacteriodetes,11.5%)以及浮霉菌门(Planctomycetex,8.5%);优势纲(>5%)为4个,包括α-变形菌纲(Alphaproteobacteria,51.7%)、δ-变形菌纲(Deltaproteobacteria,31.8%)、鞘脂杆菌纲(Sphingobacteria,9.9%)以及OM190纲(8.9%);优势属(>5%)为6个,侏囊菌科下未知属(norank Nannocystaceae,21.8%)、Pyruvatibacte属(11.6%)、Phaeodactylibacter属(9.4%)、生丝单胞菌科下未知属(norank Hyphomonadaceae,8.5%)、OM190纲下未知属(8.1%)以及Roseovarius属(7.9%)。从利玛原甲藻PL11培养物中分离获得可培养微生物菌株9株,分属于8个属,包括Ochrobactrum sp.(2株)及Microbacterium sp.、Algoriphagus sp.、Ponticoccus sp.、Hoeflea sp.、Labrenzia sp.、Sphingopyxis sp.、Erythrobacter sp.各1株。其中PL11-1为Ponticoccus属潜在新种。
As a typical red tide dinoflagellate,Prorocentrum lima has an important impact on the ecological environment and human health.However,the mechanism for the production of toxins by Prorocentrum lima is not yet conclusive.Algae-bacterial interaction(ABI)is the key to reveal DSP production mechanism.In order to reveal the information of the symbiotic and epiphytic bacteria of the typical red tide dinoflagellate,Prorocentrum lima PL11,the species and relative abundance of PL11 co-existing bacteria were analyzed by Illumina MiSeq high-throughput sequencing,and the culturable strains were selectively isolated and analyzed by the 16S rRNA gene sequence amplification and phylogeny.The high-throughput sequencing results of P.lima PL11 showed that the co-existing bacteria included 5 phyla,14 classes,26 orders,38 families and 54 genera.Among them,there were 3 dominant phyla(>5%),including Proteobacteria(75.5%),Bacteriodetes(11.5%)and Planctomycetex(8.5%).There were 4 dominant classes(>5%),including Alphaproteobacteria(51.7%),Deltaproteobacteria(31.8%),Sphingobacteria(9.9%),and OM190(8.9%).There were 6 dominant genus(>5%),norank gene of Nannocystaceae(21.8%),including Pyruvatibacter sp.(11.6%),Phaeodactylibacter sp.(9.4%),unknown genus of Hyphomonadaceae(8.5%),unknown genus of OM190(8.1%)and Roseovarius sp.(7.9%).Nine strains were isolated and classified into eight genera from the culture of PL11,among which were two Ochrobactrum sp.,and each one of Microbacterium sp.,Algoriphagus sp.,Ponticoccus sp.,Hoeflea sp.,Labrenzia sp.,Sphingopyxis sp.,and Erythrobacter sp..Among them,PL11-1 is a potential new species of Ponticoccus sp..These 9 strains of culturable bacteria were obtained by pure culture method,which provided necessary biomaterials for further study on the role of bacteria in the production of toxins by microalgae.The strain PL11-1 is closely related to the red tide outbreak of algae,which may inhibit the growth of many red tide algae,and may have a close relationship with the growth and production of dinoflagellate.The strain fan3-1 can degrade microcystins.Further study of the interaction between PL11-1 and fan3-1 with Prorocentrum lima can better understand the mechanism of toxin production and degradation.
作者
李月月
田晓清
韩清华
樊成奇
马丽艳
陆亚男
LI Yue-yue;TIAN Xiao-qing;HAN Qing-hua;FAN Cheng-qi;MA Li-yan;LU Ya-nan(Shanghai Ocean University,Shanghai 201306,China;East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Shanghai 200090,China;Key Laboratory of East China Sea Fishery Resources Exploitation,Ministry of Agriculture and Rural Affairs,Shanghai 200090,China)
出处
《海洋渔业》
CSCD
北大核心
2020年第1期73-81,共9页
Marine Fisheries
基金
科技基础资源调查专项(2018FY100200)
上海市科学技术委员会仪器试剂项目(15142201600)。
关键词
利玛原甲藻
腹泻性贝毒
共附生菌
高通量测序
Prorocentrum lima
diarrhetic shellfish poisoning
biodiversity
high-throughput sequencing
