摘要
通过土培和油菜盆栽试验研究了1株氧化木糖无色杆菌LAX2对Cu、Zn、Pb和Cd复合污染菜地土壤的修复作用。结果表明:菌株LAX2的发酵液对土壤中有效态Cu、Zn、Pb和Cd具有较强的固定作用,加入发酵液后能够快速固定土壤中的Cu、Zn、Pb和Cd,培养30 d后有效态Cu、Zn、Pb和Cd含量分别下降了87. 4%、68. 1%、86. 8%和89. 2%。油菜盆栽试验表明,在整个植物生长期内,菌株LAX2能固定土壤中有效态Cu、Zn、Pb和Cd,使其有效态含量分别下降了65. 4%、61. 8%、62. 1%和55. 3%;油菜可食部Cu、Zn、Pb和Cd的含量分别降低了42. 1%、34. 8%、46. 4%和30. 8%;油菜根中Cu、Zn、Pb和Cd的含量分别降低了58. 6%、47. 4%、53. 3%和39. 1%。这说明,菌株LAX2可通过生物矿化作用同时固定菜田土壤中共存的Cu、Zn、Pb和Cd,从而降低土壤中重金属的生物有效性,减少植物根部对重金属的吸收及重金属在可食部的积累,在重金属复合污染菜田土壤修复方面具有重要的应用价值。
Soil culture and pot experiment of rape were carried out to study the remediation effect of a bacterial strain Achromobacte rxylosoxidans LAX2 on vegetable soil simultaneously contaminated with Cu,Zn,Pb and Cd.The results showed that the fermentation broth of strain LAX2 had a strong fixation effect on available Cu,Zn,Pb and Cd in soil.After adding fermentation broth,the contents of available Cu,Zn,Pb and Cd in soil could be rapidly fixed.After 30 days of cultivation,the contents of available Cu,Zn,Pb and Cd decreased by 87.4%,68.1%,86.8%and 89.2%,respectively.Pot experiment on rape showed that strain LAX2 could fix available Cu,Zn,Pb and Cd in soil during the whole growth period,which reduced the contents of available Cu,Zn,Pb and Cd in soil by 65.4%,61.8%,62.1%and 55.3%.respectively.The contents of Cu,Zn,Pb and Cd in edible parts of rape decreased by 42.1%,34.8%,46.4%and 30.8%,respectively.The contents of Cu,Zn,Pb and Cd in rape roots decreased by 58.6%,47.4%,53.3%and 39.1%,respectively.These results indicate that strain LAX2 can fix the coexisting heavy metals of Cu,Zn,Pb and Cd in vegetable soil through biomineralization,thus reducing the bioavailability of heavy metals in soil and the absorption of heavy metals by plant roots and their accumulation in edible parts.It has important application value in the remediation of vegetable soil polluted with heavy metals.
作者
宋雷蕾
何佳宝
刘可为
SONG Leilei;HE Jiabao;LIU Kewei(Jilin Provincial Academy of Environmental Sciences,Changchun 130012,China)
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2019年第6期713-718,共6页
Journal of Jilin Agricultural University
基金
国家重点研发计划项目(2017YFD0801104)
吉林省自然科学基金项目(20180101084JC)。
关键词
氧化木糖无色杆菌
生物矿化
重金属复合污染
菜田土壤
Achromobacte rxylosoxidans
biomineralization
heavy metal combined pollution
vegetable soil
