摘要
以金黄色葡萄球菌(Staphylococcus aureus)ATCC 6538与人参土芽孢杆菌(Bacillus ginsengihumi)为供试菌,研究贮存时间和不同的辅助添加剂对辐照胰酪大豆胨琼脂(tryptic soy agar,TSA)培养基适用性的影响;同时,通过响应面优化高过氧化氢酶菌株Bacillus firmus GL3的粗酶发酵液,验证其对改善辐照培养基适用性的可行性。结果表明,贮存时间15 d以上或辅助添加剂焦性没食子酸、五倍子酸、L-半胱氨酸、酪氨酸、苯丙氨酸、金属离子Mg^2+、Mn^2+、K^+以及高过氧化氢酶菌株的粗酶发酵液的添加对辐照培养基中敏感菌株的恢复生长有效果;并确定B.firmus GL3在最优产酶条件下产过氧化氢酶活性提高了78.84%,优化后的粗酶液对敏感菌的促生长作用效果最佳,且与纯过氧化氢酶的作用效果基本一致。
This study is aiming to screen factors that can improve the suitability of irradiated tryptic soy agar(TSA)media.Staphylococcus aureus(S.aureus)ATCC 6538 and Bacillus ginsengihumi(B.ginsengihumi)were used to study the effects of storage time and different auxiliary additives on the applicability of irradiated TSA medium.In addition,the response surface methodology(RSM)was used to optimize the crude enzyme fermentation broth of Bacillus firmus(B.firmus)GL3.At the same time,the effects of the optimal crude enzyme fermentation broth of B.firmus GL3 on the irradiated medium were explored.The results showed that S.aureus and B.ginsengihumi resumed normal growth when the media were stored for 15 d or certain additives were added.By comparing the various auxiliary additives,pyrogallic acid,gallic acid monohydrate,L-cysteine,tyrosine,phenylalanine,metal ions Mg^2+,Mn^2+,K+and crude enzyme fermentation broth of catalase-producing strains can promote the growth of sensitive strains.Under optimal conditions,the activity of catalase produced by B.firmus GL3 was increased by 78.84%.Further,the optimal crude enzyme solution of B.firmus GL3 had the best growth-promoting effect on sensitive bacteria.This study provided reference for improving the applicability of irradiated TSA medium.
作者
徐玲
宁喜斌
XU Ling;NING Xibin(College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China;Shanghai Engineering Research Center of Aquatic-Product Processing&Preservation,Shanghai 201306,China;Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation(Shanghai),Ministry of Agriculture,Shanghai 201306,China;National R&D Branch Center for Freshwater Aquatic Products Processing Technology(Shanghai),Shanghai 201306,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2020年第4期99-105,共7页
Food and Fermentation Industries
关键词
辐照培养基
辅助添加剂
适用性
酶活性优化
irradiated medium
auxiliary additive
applicability
enzyme activity optimization
