摘要
目的构建电灼伤致创伤性局灶性胰腺炎的动物模型,模拟临床胃癌根治术后胰腺炎。方法将30只SD大鼠分为对照组和建模组,建模组使用高频电刀(模式:bipolar,功率:5 W)电凝胰腺被膜,对照组仅翻动胰腺后复位,分别在术后24、72 h和7 d 3个时间节点进行解剖和取材,提取血清、腹腔积液,切取胰腺组织并合理保存标本,使用酶联免疫吸附试验(ELISA)试剂盒检测血清和腹腔积液中的淀粉酶、磷脂酶A2,切取胰腺组织做病理切片,行HE染色后观察病理改变。结果术后72 h,建模组大鼠血清淀粉酶水平明显高于对照组[(70.94±2.14)U/L vs.(57.47±4.33)U/L,P<0.01]。建模组大鼠腹腔积液淀粉酶水平在术后24 h[(154.73±7.65)U/L vs.(31.77±2.85)U/L,P<0.01]、72 h[(125.27±8.34)U/L vs.(32.28±4.62)U/L,P<0.01]、7 d[(103.53±10.56)U/L vs.(31.26±4.13)U/L,P<0.01]均明显高于对照组。术后24 h建模组大鼠血清磷脂酶A2水平高于对照组[(7.18±0.68)ng/mL vs.(6.80±0.50)ng/mL,P<0.05];术后72 h、7 d建模组大鼠血清磷脂酶A2水平与对照组比较差异无统计学意义(P>0.05);建模组大鼠腹腔积液磷脂酶A2水平在术后24 h[(12.38±0.82)ng/mL vs.(7.74±0.52)ng/mL,P<0.01]、72 h[(11.28±0.71)ng/mL vs.(7.40±0.58)ng/mL,P<0.01]、7 d[(11.88±0.59)ng/mL vs.(7.18±0.84)ng/mL,P<0.01]均明显高于对照组。组织病理切片提示建模组的胰腺组织出现充血、水肿、局部坏死。结论创伤性局灶性胰腺炎的大鼠模型构建简便,成功率高,为进一步研究奠定了基础。
Objective To construct the animal model of traumatic focal pancreatitis by electric burn injury for simulating pancreatitis after gastric cancer radical operation in clinic.Methods Thirty SD rats were divided into the control group(C)and modeling group(M).The M group used the high frequency electrotome(mode:bipolar,power:5 W)electrocoagulation pancreas envelop,while the control group only reset after turning over the pancreas.The dissection and sampling were performed at the time points of postoperative 24,72 h,7 d.The serum and ascites were extracted.The pancreatic tissue was taken and the sample was rationally preserved.The levels of amylase and phospholipase A2 in serum and ascites were detected by using the enzyme linked immunosorbent assay(ELISA).The pancreatic tissue was taken for preparing the pathologic section.The pathologic change was observed after HE staining.Results The level of serum amylase at postoperative 72 h in the M group was significantly higher than that in the C group[(70.94±2.14)U/L vs.(57.47±4.33)U/L,P<0.01].The levels of ascites amylase at postoperative 24,72 h and 7 d in the M group were significantly higher than those in the C group[(154.73±7.65)U/L vs.(31.77±2.85)U/L,P<0.01,(125.27±8.34)U/L vs.(32.28±4.62)U/L,P<0.01,(103.53±10.56)U/L vs.(31.26±4.13)U/L,P<0.01].The level of serum phospholipase A2 at postoperative 24 h in the M group was significantly higher than that in the C group[(7.18±0.68)ng/mL vs.(6.80±0.50)ng/mL,P<0.05],which at postoperative 72 h and on postoperative 7 d had no statistically significant difference between the C group and M group(P>0.05).The levels of ascites phospholipase A2 at postoperative 24,72 h,7 d in the M group were significantly higher than those in the C group[(12.38±0.82)ng/mL vs.(7.74±0.52)ng/mL,P<0.01,(11.28±0.71)ng/mL vs.(7.40±0.58)ng/mL,P<0.01,(11.88±0.59)ng/mL vs.(7.18±0.84)ng/mL,P<0.01].The histopathological section examination revealed that the pancreatic tissue in the M group appeared hyperemia,edema and local necrosis.Conclusion The rat model construction of traumatic focal pancreatitis is simple with high success rate,which laids the foundation for further research.
作者
仲卫冬
钱雷敏
钱建忠
花晨
张婷
陈海姣
黄建明
ZHONG Weidong;QIAN Leimin;QIAN Jianzhong;HUA Chen;ZHANG Ting;CHEN Haijiao;HUANG Jianming(Department of Gastrointestinal Surgery,Jiangyin Municipal People′s Hospital,Jiangyin,Jiangsu 214400,China;Department of Pathology,Jiangyin Municipal People′s Hospital,Jiangyin,Jiangsu 214400,China;Central Laboratory,Jiangyin Municipal People′s Hospital,Jiangyin,Jiangsu 214400,China)
出处
《重庆医学》
CAS
2020年第5期809-812,共4页
Chongqing medicine
基金
江苏省无锡市卫生局面上科研项目（MS201410）
关键词
电灼伤
创伤性局灶性胰腺炎
大鼠模型
electric burn injury
traumatic focal pancreatitis
rat model
