摘要
为探讨巴氏蘑菇子实体不同发育阶段基因的表达情况,本研究对巴氏蘑菇子实体不同发育时期(原基、采收期和开伞期)进行转录组测序,以本实验室已获得的巴氏蘑菇JA菌株的不育单孢菌株JA-15036基因组为参考基因组研究原基与采收期及开伞期样本间差异表达基因,并对差异表达基因进行了GO功能和Pathway富集分析。GO功能分析结果显示,差异表达基因主要富集在跨膜转运、碳水化合物代谢途径和膜组分,它们协同调控为子实体生长发育提供稳定的内环境。KEGG富集分析结果表明,原基期上调的差异表达基因主要富集在核糖体蛋白和DNA复制,表明原基期细胞代谢旺盛,其中核糖体蛋白基因上调为后期蛋白质合成提供重要场所;采收期和开伞期子实体时期差异表达基因主要富集在碳水化合物代谢、脂肪酸降解和氨基酸代谢等途径,为巴氏蘑菇子实体的生长发育与成熟提供营养与能量。
In order to investigate the gene transcript pattern at different developmental stages of Agaricus blazei fruiting bodies(primordial stage, harvest stage and pileus opening stage), transcriptome analysis was carried out and genome sequence of sterile monospore strain JA-15036 obtained previously in our laboratory was used as reference genome. The gene ontology classification showed that the differentially expressed genes(DEGs) were mainly enriched in transmembrane transport, carbohydrate metabolism pathway and membrane components, and the coordinated regulation of these genes could provide a stable internal environment for the growth and development of fruiting bodies. KEGG pathway analysis showed that the DEGs were mainly enriched in ribosome, DNA replication, carbohydrate metabolism, fatty acid degradation and amino acid metabolism. Of these, most genes related to ribosome and DNA replication were up-regulated at primordial stage, indicating that the cell metabolism was more active in primordia, which could be prepare for protein synthesis. The up-regulated DEGs at harvested stage and pileus opening stage were mainly enriched in carbohydrate metabolism, fatty acid degradation and amino acid metabolism, implying that these genes have an important role in providing nutrition and energy for A. blazei during fruiting body development process.
作者
卢园萍
郭仲杰
蔡志欣
陈美元
廖剑华
LU Yuan-Ping;GUOZhong-Jie;CAI Zhi-Xin;CHEN Mei-Yuan;LIAO Jian-Hua(The Edible Fungal Institute of Fujian Academy of Agricultural Sciences,National and Local Joint Engineering Research Center for Breeding&Cultivation of Featured Edible Fungi,Fuzhou,Fujian 350014,China)
出处
《菌物学报》
CAS
CSCD
北大核心
2019年第12期2161-2173,共13页
Mycosystema
基金
公益性行业(农业)科研专项课题(201503137)
福建省属公益类科研院所基本科研专项(2017R1020-1)
福建省种业创新与产业化工程项目(fjzycxny2017009)
福建省农业科学院科技创新团队(STIT2017-1-6)~~
关键词
巴氏蘑菇
生长发育
子实体
转录组
Agaricus blazei
growth and development
fruiting body
transcriptome
