摘要
[目的]以微晶纤维素为底物,从宝天曼自然保护区采集的腐木和土壤中筛选产纤维素酶活高的真菌,并优化其培养基配方。[方法]通过微晶纤维素平板上产生的透明圈大小进行初筛,和滤纸酶活复筛,并通过响应面法优化其发酵培养基。[结果]筛选到一株酶活较高的菌株,经18S r DNA序列分析鉴定为哈茨木霉,命名为哈茨木霉D-8,通过响应面分析法优化后的培养基配方为木糖渣2.86%,麸皮2%,微晶纤维素0.48%,(NH4)2SO40.32%,KH2PO41%,MgSO40.04%。[结论]滤纸酶活从优化前的4.32 IU/m L提高到5.53 IU/m L,使其纤维素酶活提高了28%,其培养基的优化为哈茨木霉D-8的发酵生产奠定了应用基础。
[Objective]The fungi with high cellulose producing ability for degradation of microcrystalline cellulose from rotten wood and soil collected in Baotianman nature reserves were screened and the culture medium were optimized.[Method]The samples were first screened with transparent zone on the medium and analyzed by filter paper enzyme activity(FPA)for re-screened.Response surface methodology was adopted to optimize the culture medium.[Result]One strain with high cellulase activity was screened and named as Trichoderma harzianum D-8 after sequencing its 18S rDNA.The culture medium was optimized to xylose residue 2.86%,bran 2%,microcrystalline cellulose 0.48%,(NH4)2 SO40.32%,KH2PO41%,MgSO40.04%by response surface method.[Conclusion]At these conditions,the FPA activity of this strains was increased 28%from 4.32 IU/mL to 5.53 IU/mL which laid a foundation for the fermentation of T.harzianum D-8.
作者
张家麟
柯涛
王曦
王进
ZHANG Jia-lin;KE Tao;WANG Xi;WANG Jin(College of Life Science and Technology,Nanyang Normal University,Nanyang 473000,China)
出处
《生物技术》
CAS
2019年第6期566-572,共7页
Biotechnology
基金
国家自然科学基金项目(31570021)
河南省教育厅科学技术研究重点项目基础研究计划(18A180025)
河南省科技厅攻关项目(182102310654)
南阳师范学院大学生实践教学活动创新项目(2019)
关键词
哈茨木霉
培养基
优化
纤维素酶
滤纸(FPA)酶活
响应面
Trichoderma harzianum
culture medium
optimize
cellulase
filter paper enzyme activity
response surface method
