摘要
目的探讨miR-141-3p在卵巢癌细胞对顺铂耐药中的表达和作用。方法采用实时荧光定量PCR法检测A2780/DDP细胞中miR-141-3p的表达水平;下调/过表达miR-141-3p后,采用CCK-8法检测卵巢癌细胞对顺铂的增殖率并计算药物IC50;采用Western blot法检测增殖相关蛋白(proliferating cell nuclear antigen,PCNA)表达水平。结果miR-141-3p在顺铂耐药的卵巢癌细胞株A2780/DDP中的表达显著高于顺铂敏感的卵巢癌细胞株A2780(P<0.05)。与对照组比较,A2780细胞中过表达miR-141-3p后可显著增加细胞对顺铂的IC50,增殖相关蛋白PCNA表达亦增加(P<0.05)。与对照组比较,A2780/DDP细胞中下调miR-141-3p表达后,细胞对顺铂的IC50显著下降,增殖相关蛋白PCNA表达下降(P<0.05)。结论miR-141-3p在顺铂耐药卵巢癌细胞的耐药机制中表达增加,下调miR-141-3p可增加顺铂耐药的卵巢癌细胞对顺铂的敏感性。
Objective To investigate the expression and role of miR-141-3p in Cisplatin resistance of ovarian cancer cells.Methods RT-qPCR was performed to identify the expression of miR-141-3p in A2780/DDP cells.CCK8 assay was used to exam cell proliferation.Western blot analysis was used to detect proliferating cell nuclear antigen(PCNA)protein level.Results miR-141-3p was upregulated in DDP-resistance A2780/DDP cells compared with DDP-sensitive A2780 cells(P<0.05).Overexpression of miR-141-5p in A2780 cells increased the IC50 of DDP compared with negative control,and also increased PCNA protein expression(P<0.05).Knockdown of miR-14-5p in A2780/DDP cells decreased the IC50 of DDP compared with negative control,and also decreased PCNA protein expression(P<0.05).Conclusion The expression of miR-141-3p increased in DDP-resistance of ovarian cancer.Silencing of miR-141-3p can increase the chemo-sensitivity in ovarian cancer cells to DDP.
作者
穆卫红
陈文琪
彭园园
张静
MU Wei-hong;CHEN Wen-qi;PENG Yuan-yuan;ZHANG Jing(Department of Prenatal Diagnosis Center,Shijiazhuang Obstetrics and Gynecology Hospital,Hebei Province,Shijiazhuang 050011,China)
出处
《河北医科大学学报》
CAS
2020年第2期167-171,共5页
Journal of Hebei Medical University
关键词
卵巢肿瘤
抗药性
肿瘤
顺铂
ovarian neoplasms
drug resistance
neoplasms
cisplatin
