摘要
靛蓝是历史上最早发现,被广泛应用于印染、医药和食品等行业的天然染料之一。该实验室分离纯化得到一株能以吲哚为唯一碳源生长且具有转化吲哚合成靛蓝能力的菌株IDO,经16S rRNA基因序列分析鉴定为贪铜杆菌(Cupriavidus)。该研究进一步对该菌株合成靛蓝的条件进行优化,考察了外加金属离子、外加芳香化合物以及焦化废水中常见污染物等不同环境因素对其合成靛蓝特性的影响。结果表明,菌株IDO合成靛蓝的最佳条件为:温度30℃,转速150 r/min,培养基pH为7。在最优条件下,该菌株能在15 h内完全转化100 mg/L吲哚,并生成30 mg/L左右的靛蓝。实验证明Fe^3+对靛蓝合成起促进作用,而萘及喹啉会明显抑制靛蓝的合成。菌株IDO能够转化4-甲基吲哚、5-甲基吲哚、7-甲基吲哚等吲哚同系物并合成相应的靛蓝类色素。该研究在微生物合成靛蓝方面提供了高效新颖的菌株资源,为靛蓝类色素的生物合成奠定理论基础。
Indigo is one of the oldest natural dyes,widely used in dyeing,medicine and food industries.A novel indole degrading strain IDO was isolated from soil sample and identified as Cupriavidus sp.,according to 16S rRNA sequence analysis,and it could utilize indole as the sole carbon and transform indole into indigo.In this study,the conditions for the synthesis of indigo were further optimized,and the effects of different environmental factors like metal ions,aromatic compounds and typical compounds in coking wastewater during the synthesis process of indigo by the strain IDO were investigated.The results indicated that the optimal conditions for indigo production were temperature 30℃,rotary speed 150 r/min and pH 7.Under the optimal conditions,strain IDO could synthesize 30 mg/L indigo from 100 mg/L indole in 15 h.Indigo production rate was promoted after induced by Fe^3+,while both the naphthalene and quinolone had obvious inhibiting effects.In addition,the strain IDO was able to convert 4-methylindole,5-methylindole,7-methylindole and other indole derivatives into indigo pigments.In a word,this research provides highly efficient and novel bacterial resources for the microbial synthesis of indigo and lays a theoretical foundation for the biosynthesis of indigo pigments and the bioremediation of indole containing wastewater.
作者
冉傈予
吕阳
朴世元
由胜男
戴春晓
周端迪
李严
马桥
曲媛媛
RAN Liyu;LYU Yang;PIAO Shiyuan;YOU Shengnan;DAI Chunxiao;ZHOU Duandi;LI Yan;MA Qiao;QU Yuanyuan(Key Laboratory of Industrial Ecology and Environmental Engineering of Ministry of Education,School of Environmental Science and Technology,Dalian University of Technology,Dalian 116024,China;Institute of Environmental Systems Biology,College of Environmental Science and Engineering,Dalian Maritime University,Dalian 116026,China)
出处
《环境科学与技术》
CAS
CSCD
北大核心
2019年第10期88-94,共7页
Environmental Science & Technology
基金
哈尔滨工业大学城市水资源与水环境国家重点实验室开放课题（QAK201943）
