摘要
目的探讨miR-134在癫痫患者外周血中是否存在差异表达、及其表达的意义。方法使用Trizol提取癫痫患者血清中RNAs,进行逆转录及实时荧光定量PCR检测样本中miR-134表达量,根据病程、发作时限、发作频率及特殊癫痫综合征将病例进行亚组分析。结果与对照组相比,癫痫组外周血miR-134表达量显著升高;在长病程组及急性发作组中明显升高。在不同发作频率组、特殊癫痫综合征组中无差异表达。结论miR-134在癫痫患者外周血中表达量与病程、发作时限相关,提示miR-134表达与神经元放电程度和损伤严重性相关,可以成为癫痫血清学标志物进行深入研究。
Objective To investigate the difference and significance in the expression of miR-134 in peripheral blood of patients with epilepsy.Methods Trizol was used to extract serum RNAs of patients with epilepsy,NCode TM VILO TM micrornas cDNA short Kit and Express Sybr those TM micrornas qRTPCR Kits was used to reverse transcriptions and real-time fluorescent quantitative PCR to detect the expression of miR-134.All cases were grouped according to the course of disease,seizure frequency,onset time limit,brain electrical activity and special epilepsy syndrome.Results Compared with control group,the expression of miR-134 in peripheral blood of epilepsy group is significantly increased,it is obviously higher in long course group and acute group.The expression of miR-134 in different seizure frequency group and special epilepsy syndrome group has no difference.Conclusion The expression of miR-134 in peripheral blood of patients with epilepsy was significantly increased while it was affected by the length of disease and the duration of seizure.In addition,the expression of miR-134 in peripheral blood of patients with epilepsy was positively correlated with the degree of discharge and severity of damage,indicating that miR-134 could be a biomarker for epilepsy.
作者
王晓爽
国琦
朱亚飞
樊雪梅
刘松岩
WANG Xiaoshuang;GUO Qi;ZHU Yafei(Department of Neurology,China-Japan Union Hospital of Jilin University,Changchun 130033,China)
出处
《中风与神经疾病杂志》
CAS
2020年第2期154-157,共4页
Journal of Apoplexy and Nervous Diseases
基金
吉林省科技发展计划项目(20180311075yy)
吉林省卫生科研人才专项项目(2019scz044)。
