摘要
建立超高效液相色谱法(ultra performance liquid chromatography,UPLC)同时测定橘核中7种成分(柚皮素、橙皮素、柚皮苷、橙皮苷、柠檬苦素、诺米林和黄柏酮)含量。采用UHPLC Polar-AQ-C18色谱柱(2.1 nm×100 mm,1.8μm),以乙腈(A)-水(B)为流动相,流速0.21 mL/min进行梯度洗脱,柱温30℃,检测波长为283 nm(0~20 min)、210 nm(20 min^35 min)。结果显示,7种成分均达到基线分离,各成分均有较宽的线性范围和良好的线性关系(r≥0.9999),方法精密度、重复性、稳定性良好,平均加样回收率在102.62%~109.18%,相对标准偏差(relative standard deviation,RSD)均<2%。16批橘核样品中7种成分含量差异较大,其中柠檬苦素和诺米林的含量最高。
To establish an ultra performance liquid chromatography(UPLC)method for the simultaneous determination of seven constituents(naringenin,hesperetin,naringin,hesperidin,limonin,nomilin,obakunone.)in Citri reticulatae semen.The separation was performed on a UHPLC Polar-AQ-C18(2.1 nm×100 mm,1.8μm)was used as the stationary phase and the mobile phase consisted of acetonitrile(A)-water(B)with gradient elution at flow rate of 0.21 mL/min,the detection wavalength at 283 nm(0-20 min)and 210 nm(20 min-35 min),and the column temperature at 30℃.The results showed that the seven components reached baseline separation all the compounds had a wide linear range and good linearity(r≥0.9999),with good precision,repeatability and stability.The average recoveries were between 102.62%-109.18%and the relative standard deviation(RSD)were all less than 2%.The contents of seven components in 16 batches of Citri reticulatae semen were different and the contents of limonin and nomilin were the highest.
作者
刘兴赋
郁林娜
付洋
程盛勇
鲁文琴
罗喜荣
杨军
LIU Xing-fu;YU Lin-na;FU Yang;CHENG Sheng-yong;LU Wen-qin;LUO Xi-rong;YANG Jun(School of Pharmaceutical Sciences,Guizhou Medcial University,Guiyang 550025,Guizhou,China;The Affiliated Hospital of Guizhou Medical University,Guiyang 550004,Guizhou,China;Institute of Geochemistry,Chinese Academy of Sciences,Guiyang 550081,Guizhou,China)
出处
《食品研究与开发》
CAS
北大核心
2020年第3期179-182,201,共5页
Food Research and Development
基金
贵州省科学技术基金项目（黔科合J字[2013]2037号）
贵州省科技计划项目（黔科合SY字[2015]3032号、黔科合LH字[2014]7091号）
关键词
橘核
超高效液相色谱
含量测定
质量控制
波长切换
Citri reticulatae semen
ultra performance liquid chromatograph
content determination
quality control
switching wavelength
