摘要
目的观察高脂饮食诱导的肥胖大鼠肝脏病理形态学改变并讨论其意义。方法用高脂饮食诱导成肥胖大鼠12只(实验组,Ob组),同时设对照组(Con组)8只。共观察14周。分别检测血糖、血脂、血尿酸、胰岛素。计算体重指数,稳态模型评估法(H0MRA-IR)估测组织胰岛素抵抗及计算胰岛素敏感性指数(ISI)。肝脏组织进行HE染色。结果Ob组与Con组各基线参数比较,包括性别、体重、身长、尾长,Lee's指数等差异无统计学意义(P>0.05),但14周时Ob组体重、身长,Lee's指数明显高于Con组[(367.92±88.88)g vs(252.5±59.47)g,(23.14±2.18)cm vs(20.81±1.71)cm,(328.18±9.82)vs(265.00±38.17),(P<0.05)]。Ob组与Con组比较空腹血糖升高,高密度脂蛋白降低[(7.00±1.81)mmol/L vs(4.21±1.13)mmol/L,(1.24±0.14)mmol/L vs(1.41±0.15)rmnol/L,(Pv0.05)]。血清胰岛素,甘油三酯、总胆固醇和低密度脂蛋白,血尿酸两组间差异无统计学意义。Ob组与Con组比较HOMA-IR及ISI均无明显差异,0b组大鼠肝脏组织光镜下可见气球样变及脂肪变性。结论肥胖大鼠肝脏组织光镜下可见气球样变及脂肪变性,同时伴有血糖升高及高密度脂蛋白降低,是肥胖导致脂肪肝及糖脂代谢异常的基础。
Objective To observe the liver histology change of obesity rat induced by high fat diet and discuss its significance.Methods The rats were divided into 2 groups:12 obesity rats induced by high fat diet and 8 rats for control.Then the fasting blood glucose,lipid,blood uric acid,insulin level were tested while BMI,HOMA-IR and ISI were calculated at 14 week.Also the HE staining of the liver tissue was performed.Results The baseline parameters including gender,body weigh,body length,tail length and Lee's Index were similar between the two groups(P>0.05).But after 14 weeks,the body weigh,body length and Lee's Index of the obesity rats were higher significantly than the normals[(367.92±88.88)g vs(252.5±59.47)g,(23.14±2.18)cm vs(20.81±1.71)cm,(328.18±9.82)vs(265.00±3&17),(Pv0.05)].The fasting blood glucose of obesity group was higher than that of control group,but the HDL level of obesity group was lower than that of control group[(7.00±1.81)mmol/L vs(4.21±1.13)mmol/L,(1,24±0.14)mmol/L vs(1.41±0」5)mmol/L,(P<0.05)].The insulin,triglycerides,cholesterol,LDL,urimic acide,HOMA-IR and ISI were not different between the two groups.The balloon degeneration and fatty degeneration of the liver tissue were observed under light microscope at the obesity group.Conclusion The balloon-like changes and steatosis of the liver tissue,as well as the high blood sugar and high-density lipoprotein,were the basis of obesity induced abnormal metabolism including fatty liver and glycolipids.
作者
杜娟
朱安峰
宋东明
米旭光
姜洪波
Du Juan;Zhu Anfeng;Song Dongming;Mi Xuguang;Jiang Hongbo(Department of Endocrinology,the People’s Hospital of Jilin Province,Changchun 130021,China;Department of Pathology,Changchun Central Hospital,Changchun 130021,China;Central Laboratory,the People’s Hospital of Jilin Province,Changehunl30021,China;laboratory,Changchun Children's Hospital,Changchun130021,China)
出处
《中华内分泌外科杂志》
CAS
2019年第6期463-465,共3页
Chinese Journal of Endocrine Surgery
关键词
肥胖
大鼠
胰岛素抵抗
病理学
Obesity
Rat
Insulin resistance
Pathology
