摘要
目的观察过敏性鼻炎患儿肠道菌群变化及双歧杆菌三联活菌散对其辅助治疗的效果。方法选取我院就诊的过敏性鼻炎患儿60例,从中随机抽取10例为疾病组,并以10例体检的健康儿童为健康对照组,采集两组对象粪便标本,用高通量测序法对比过敏性鼻炎患儿与健康对照组肠道菌结构的差异。另一方面,将入选的60例过敏性鼻炎患儿分为对照组(30例,采用常规治疗)和治疗组(30例,常规治疗联合双歧杆菌三联活菌治疗)。两组患儿连续治疗8周后,观察其治疗前后临床症状评分及血清IL-4、IL-12、IgE水平变化。结果过敏性鼻炎患儿肠道菌群多样性低于健康对照组。患儿肠道菌群以拟杆菌门为主,健康对照组以厚壁菌门为主(PV0.05)。患儿肠道双歧杆菌属数量低于健康对照组,而毛螺菌属,瘤胃菌属,红蜻菌属和普雷沃菌属有所增多(均PV0.05)。治疗8周后,治疗组患儿临床症状评分明显降低,治疗的总有效率高于对照组(PV0.05)。治疗组患儿血清IL-4.IgE水平明显降低,IL-12水平明显升高,且治疗组患儿以上指标的改善程度均优于对照组(均PV0.05)。结论过敏性鼻炎患儿与健康儿童相比肠道菌群存在差异.肠道菌群失衡可能是促进过敏性鼻炎发生发展的一个因素。双歧杆菌三联活菌散对过敏性鼻炎有辅助治疗的作用,能显著改善患儿临床症状,调节机体的免疫能力,具有一定临床应用价值。
Objective To discuss the change of intestinal flora of children with allergic rhinitis(AR) and the adjuvant therapeutic effect of Bifidobacterium Triple Viable. Methods 60 children with allergic rhinitis admitted to the department of ophthalmology and otolaryngology in our hospital were selected, 10 of whom were randomly selected as AR group. 10 normal children underwent physical examination were selected as healthy controls. Fecal samples were collected and high-throughput sequencing was used to compare and analyze the differences in intestinal flora between patients with allergic rhinitis and healthy controls. On the other hand, the 60 children with allergic rhinitis were divided into control group(30 cases, conventional treatment) or treatment group(30 cases, Bifidobacterium Triple Viable combined conventional treatment). After 8 weeks of continuous treatment, the clinical symptom scores and serological indexes(IL-4, IL-12 and IgE) in the two groups were compared. Results The intestinal flora diversity in AR group was lower than that in the healthy control group. The intestinal flora in AR group was dominated by Bacteroidetes, while that in the healthy control group was dominated by Firmicutes(P<0.05). Compared with the healthy control group, the number of Bifidobacteriaceae in AR group decreased, while those of Lachnospiraceae, Ruminococcaceae, Coriobacteriaceae and Prevotellaceae increased(all P<0.05). After 8 weeks of treatment, the clinical symptom scores of children in AR group significantly decreased and the total clinical efficacies was higher than that in the control group(P<0.05). Serum levels of IL-4 and IgE significantly reduced, while that of IL-12 significantly increased, and the improvement of these indicators in AR group was better than that in the control group(all P<0.05). Conclusion There are differences in intestinal flora between children with allergic rhinitis and healthy controls, and the imbalance of intestinal flora may be an important factor to promote the development of allergic rhinitis. Viable Bifidobacterium-Lactobacillus-Enterococcus Powder has an adjuvant therapeutic effect on children with allergic rhinitis, which can significantly improve clinical symptoms, regulates the body′s immune capacity, and has a certain clinical application value.
作者
陶雪莹
岑超
何俐莹
TAO Xueying;CEN Chao;HE Liying(Department of Ophthalmology and Otorhinolaryngology,Chongqing Health Center for Women and Children,Chongqing 401120,China)
出处
《中国微生态学杂志》
CAS
CSCD
2019年第12期1409-1412,共4页
Chinese Journal of Microecology
基金
重庆市科学技术委员会重点项目(cstc2017shms-zdyfX0049)
关键词
过敏性鼻炎
肠道菌群
益生菌
Allergic rhinitis
Intestinal flora
Probiotics
