摘要
目的研究海洋特色资源--瘤背石磺Onchidium verruculatum的化学成分。方法采用硅胶、半制备高效液相色谱、薄层色谱(TLC)检识等方法进行分离纯化,通过NMR、MS等波谱方法对化合物的结构进行鉴定,并结合MTT法测定化合物对人肝癌细胞HepG-2、BEL-7042、人肺腺癌细胞A549和人乳腺癌细胞MCF-7的细胞毒活性。结果从瘤背石磺的黏液及肌肉组织中分离出1个新的酰胺类化合物,N-(2-羟基-4-甲氧基-5-甲基苯基)甲酰胺(1),化合物1对A549和BEL-7402细胞具有一定的细胞毒活性,半数抑制浓度(IC50)值分别为11.5、19.4μg/mL。结论化合物1为1个新的酰胺类化合物,命名为石磺甲酰胺,且对人肿瘤细胞具有一定的细胞毒活性。
Objective To study the chemical composition of the marine characteristic resource,Onchidium verruculatum from Hainan Island.Methods Compound was separated and purified by silica gel and semi-preparative HPLC,detected by TLC,and identified by NMR and MS data.Compound 1 was evaluated for cytotoxic activity against four kinds of human tumor cell lines HepG-2,BEL-7042,A549,and MCF-7.Results A new amide compound,N-(2-hydroxy-4-methoxy-5-methylphenyl)formamide(1),was isolated from mucus and muscle tissue of O.verruculatum.The results showed that compound 1 has moderate toxic activity against A549 and BEL-7402 cell lines,with IC50 value of 11.5μg/mL and 19.4μg/mL.Conclusion Compound 1 is a new amide compound named onchumamide with moderate toxic activity on human tumor cells.
作者
王博闻
陈德力
马国需
刘洋洋
许旭东
刘平怀
WANG Bo-wen;CHEN De-li;MA Guo-xu;LIU Yang-yang;XU Xu-dong;LIU Ping-huai(School of Chemical Engineering and Technology,Hainan University,Haikou 570228,China;Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine,Hainan Branch Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences&Peking Union Medical College,Haikou 570311,China;Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100193,China)
出处
《中草药》
CAS
CSCD
北大核心
2019年第23期5666-5669,共4页
Chinese Traditional and Herbal Drugs
基金
海南省自然科学基金面上项目（219MS101）
海南省科协青年科技英才创新计划项目（201517）
关键词
软体动物
瘤背石磺
酰胺
细胞毒活性
石磺甲酰胺
mollusc
Onchidium verruculatum Cuvier
amide
cytotoxic activity
onchumamide
