摘要
本研究合成了D-甘露糖修饰的两亲性β-环糊精(C3-CD-Man7)和三臂金刚烷共价键连的BODIPY光敏剂(BTA),并利用β-环糊精(β-CD)与金刚烷(Ad)之间超分子主客体识别作用制备表面D-甘露糖修饰的高负载光敏剂胶束(BTA@C3-CD-Man7)。利用透射电镜和动态光散射对BTA@C3-CD-Man7胶束形貌、粒径和稳定性进行表征;并采用MTT细胞毒性评价方法考察其对人乳腺癌MDA-MB-231细胞的摄入、光动力治疗效果和光毒性。结果表明BTA@C3-CD-Man7粒径均一,在溶液中稳定性良好;甘露糖受体过度表达的乳腺癌细胞MDA-MB-231能够特异性识别并大量摄取BTA@C3-CD-Man7,并在665 nm LED灯照射下表现出对MDA-MB-231细胞靶向光动力治疗的效果。
In this paper,the D-mannose-modified amphiphilicβ-cyclodextrin(C3-CD-Man7)and a three-arm adamantine-conjugated BODIPY photosensitizer(BTA)are synthesized.Because of the presence of supramolecular host-vip interactions betweenβ-cyclodextrin and adamantane,BTA is loaded into the hydrophobic cavity of micelles self-assembled from C3-CD-Man7,leading to the formation of BTA-loaded micelles(BTA@C3-CD-Man7).The size,morphology,and stability of BTA@C3-CD-Man7 are characterized by transmission electron microscopy and dynamic light scattering.The cellular uptake,PDT effect,and phototoxicity of BTA@C3-CD-Man7 are investigated using the MTT assay.The results show that BTA@C3-CD-Man7 has uniform size distribution and excellent stability in aqueous solution.More importantly,BTA@C3-CD-Man7 could be specifically internalized by MDA-MB-231 breast cancer cells that overexpress mannose receptors on cell surfaces.Thus,with 665 nm LED irradiation,the enhanced phototoxicity of BTA@C3-CD-Man7 showed their potential for targeted photodynamic therapy applications.
作者
蔡颖
王胜涛
魏朋
张权
尹健
CAI Ying;WANG Shengtao;WEI Peng;ZHANG Quan;YIN Jian(Key Laboratory of Carbohydrate Chemistry&Biotechnology,Ministry of Education,Jiangnan University,Wuxi 214122,China;School of Biotechnology,Jiangnan University,Wuxi 214122,China)
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2019年第10期43-51,共9页
Journal of Food Science and Biotechnology
基金
国家自然科学基金项目(51403081)
江苏省自然科学基金项目(BK20140137)
关键词
D-甘露糖
主客体识别
载光敏剂胶束
受体介导胞吞
靶向光动力治疗
D-mannose
host-vip recognition
BTA-loaded micelles
receptor-mediated endocytosis
targeted photodynamic therapy
