摘要
目的研究布朗卷柏(Selaginella braunii Baker)中的黄酮类成分。方法采用正相硅胶柱、Sephadex LH-20柱色谱及半制备高效液相色谱等分离方法对布朗卷柏的75%乙醇提取物进行分离纯化,并根据化合物的理化性质和波谱数据进行结构鉴定。结果从布朗卷柏的75%乙醇提取物中分离得到10个黄酮类化合物,分别鉴定为8’’-methylnaringeninyl-(4’’’,O,3)-kaempferol(1)、2’’,3’’-dihydroochnaflavone(2)、白果黄素(3)、穗花杉双黄酮(4)、kaempferol-3,7-di-O-β-D-glucopyranoside(5)、芹菜素6,8-二-C-β-D-葡萄糖苷(6)、芹菜素6-C-β-D-葡萄糖-8-C-α-L-阿拉伯糖苷(7)、4’-羟基-5-羧甲基黄酮-7-O-β-D-葡萄糖苷(8)、7,4’-二羟基-5-羧甲基黄酮(9)、山奈酚(10)。结论共得到10个化合物,且10个化合物均为首次从布朗卷柏中分离得到。
Objective To study the chemical constituents of flavonoids in Selaginella braunii Baker.Methods The constituents were isolated and purified by column chromatographies on silica gel column,Sephadex LH-20 column and Prep-HPLC chromatographic methods,and their structures were elucidated by chemical and spectroscopic methods.Results Ten compounds were identified as 8’’-methylnaringeninyl-(4’’’,O,3)-kaempferol(1),2’’,3’’-dihydroochnaflavone(2),bilobetin(3),amentobiflavonoid(4),kaempferol-3,7-di-O-β-D-glucopyranoside(5),apingenin 6,8-di-C-β-D-glucopyranoside(6),apingenin 6-C-β-D-glucopyranosyl-8-α-L-arabinopyranoside(7),4’-hydroxy-5-carboxymethyl-flavonoid-7-O-β-D-glucopyranoside(8),7,4’-hydroxy-5-carboxymethyl-flavonoid(9),kaempferol(10).Conclusion Compounds 1-10 were all isolated from this plant for the first time.
作者
曲建博
吴威
肖玲
孙惠惠
朱晖
邹振兴
张燕
陈思为
刘恒宇
周辉
徐康平
QU Jianbo;WU Wei;XIAO Ling;SUN Huihui;ZHU Hui;ZOU Zhenxing;ZHANG Yan;CHEN Siwei;LIU Hengyu;ZHOU Hui;XU Kangping(Center for Drug Evaluation,National Medical Products Administration,Beijing,100022,China;Xiangya School of Pharmaceutical Sciences,Central South University,Changsha,Hunan,410013,China;School of Basic Medical Science,Central South University,Changsha,Hunan,410013,China;Department of Lymphoma Hematology,Hunan Cancer Hospital,Changsha,Hunan,410013,China)
出处
《肿瘤药学》
CAS
2019年第6期859-864,共6页
Anti-Tumor Pharmacy
基金
国家自然科学基金(31370370)
湖南省中医药科研计划(201768)
湖南省普通高等学校教学改革研究项目/中南大学深化创新创业教育改革研究项目(2019CG006)
湖南省研究生科研创新项目(CX20190247)
长沙市科技计划(kq1801072)
中南大学研究生自主探索创新项目(2019zzts752)
关键词
布朗卷柏
化学成分
黄酮类
Selaginella braunii Baker
Chemical constituent
Flavonoids
