摘要
目的:探讨黄体酮在裸鼠血管瘤发生发展中的作用。方法:体外构建裸鼠血管瘤模型,实验组用不同剂量(低、中、高剂量组分别为0.2,0.4,0.8 mg/d)黄体酮连续肌肉注射1周,对照组用茶油0.04 mL/d肌肉注射,动态观察血管瘤大小。用免疫组织化学方法检测血管内皮生长因子(vascular endothelial growth factor,VEGF)的表达和微血管密度(microvessel density,MVD)。结果:在成瘤第14天,与对照组和黄体酮低剂量组比较,黄体酮高剂量组瘤体体积增大(P<0.01),VEGF的表达水平升高(分别P<0.01和P<0.05),MVD增大(均P<0.01);在成瘤第28天,各组间瘤体体积比较差异无统计学意义(P>0.05),黄体酮中、高剂量组VEGF的表达水平较对照组和黄体酮低剂量组降低(均P<0.01),MVD较对照组和黄体酮低剂量组减小(均P<0.05)。结论:在成瘤早期,高浓度黄体酮能上调VEGF的表达水平从而促进血管生成,刺激瘤体生长;提示过量补充外源性黄体酮可能是早期血管瘤形成的始动因素之一。
Objective:To explore the role of progesterone in the pathogenesis and development of hemangioma in nude mice.Methods:The hemangioma model was established.Progesterone was injected intramuscularly at different doses (0.2,0.4,and 0.8 mg/d) for one week.Camellia oil (0.4 mL/d) was injected intramuscularly as control.The size of hemangioma was observed dynamically.The subcutaneous implants were harvested on the 14th and 28th days.The expression of vascular endothelial growth factor in the tumor tissues were evaluated using immunohistochemistry and microvessel density (MVD) was counted under the microscope.Results:On the 14th day,the expression of vascular endothelial growth factor (P<0.01 and P<0.05,respectively) was higher,the volume of tumors (All P<0.01) and MVD (All P<0.01) were greater in the high-dose progesterone group than those in the control and low-dose progesterone group.On the 28th day,there was no significant difference in the volume of tumors among 4 groups (P>0.05).The expression of vascular endothelial growth factor (P<0.01) was lower,and MVD (All P<0.05) were less in the middle-dose and high-dose progesterone group than those in the control and low-dose progesterone group.Conclusion:Progesterone promotes angiogenesis via upregulation of vascular endothelial growth factor expression,promotion of hemangiomas proliferation,suggesting that excessive progesterone supplementation may be one of the initial factors for early hemangioma formation.
作者
石青梅
陈星帆
邱凤贞
雷少榕
SHI Qingmei;CHEN Xingfan;QIU Fengzhen;LEI Shaorong(Department of Plastic Surgery,Xiangya Hospital,Central South University,Changsha 410008,China)
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2019年第12期1353-1359,共7页
Journal of Central South University ：Medical Science
关键词
血管瘤
裸鼠
动物模型
黄体酮
血管内皮生长因子
CD31
hemangioma
nude mouse
animal model
progesterone
vascular endothelial growth factor
CD31
