摘要
目的:通过小鼠菌群失调模型研究藿香正气水调节肠道菌群失调的作用。方法:按7.2 mg·g^-1剂量灌胃给予小鼠盐酸林可霉素,每天2次,连续5 d,建立小鼠肠道菌群失调模型。将造模成功的小鼠随机分为藿香正气水组、乳酶生组、模型对照组,另选取健康小鼠作为空白对照组。藿香正气水组小鼠分别灌胃3.0 mL·kg^-1、1.5 mL·kg^-1、0.75 mL·kg^-1的藿香正气水溶液,乳酶生组小鼠灌胃0.132 mg·g^-1,2次/d,连续给药7 d。模型组、空白对照组小鼠灌胃同体积的生理盐水。于末次给药12 h后取小鼠的新鲜粪便,检测肠道菌群(肠杆菌、肠球菌、双歧杆菌和乳杆菌)的变化。结果:藿香正气水高、中、低剂量组小鼠肠道的有益菌——双歧杆菌和乳杆菌数量明显上升;中、高剂量组与模型对照组比较,差异具有统计学意义(P<0.05),与空白对照组比较,差异无统计学意义(P>0.05)。结论:藿香正气水能扶植肠道正常菌群的生长,促进有益菌(双歧杆菌和乳杆菌)的增殖,具有调节肠道菌群失调的作用。
Objective To study the role of Huoxiangzhengqishui in regulating the imbalance of intestinal microflora.Methods To establish an intestinal flora imbalance model in mice,the mouse was intragastrically administrated lincomycin hydrochloride at a dose of 7.2 mg·g^-1,twice a day for 5 consecutive days.The successful model mice were randomly divided into HuoxiangZhengqiShui group,lactase group,model control group,and another healthy mice as blank control group.Huoxiangzhengqishui group were ig 3.0 kg·kg^-1,1.5 mL·kg^-1,0.75 mL·kg^-1 of Huoxiangzhengqishui aqueous solution,lactation group were ig 0.132 mg·g^-1,2 times a day,continuous administration 7 d.Model group and control group were ig the same volume of saline.The fresh stools of mice were taken 12 hours after the last administration,and the changes of intestinal flora(Enterobacteriaceae,Enterococcus,Bifidobacterium and Lactobacillus)were detected.Results The number of beneficial bacteria——Bifidobacterium and Lactobacillus in intestinal tract were significantly increased in Huoxiangzhengqishui high,medium and low dose groups;the middle and high dose groups were significantly different from the model control group(P<0.05),Compared with the blank control group,there was no significant difference(P>0.05).Conclusion Huoxiangzhengqishui can support the growth of normal intestinal flora and promote the proliferation of beneficial bacteria(Bifidobacterium and Lactobacillus),which can regulate the imbalance of intestinal flora.
作者
杨润
叶志亮
陈崇莉
YANG Run;YE Zhiliang;CHEN Chongli(Department of Gastroenterology,Ganzi Tibetan Autonomous Prefecture People’s Hospital,Kangding 62600,China;Affiliated Hospital of Chengdu University of Chinese Medicine,Chengdu 61105,China)
出处
《中国民族民间医药》
2019年第24期34-36,共3页
Chinese Journal of Ethnomedicine and Ethnopharmacy
基金
四川省干部保健科研课题(川干研2016-506)
关键词
藿香正气水
菌群失调
藿香
Huoxiangzhengqishui
Flora imbalance
Agastache rugosa
